The development of pcr system for identification of beet necrotic yellow vein virus and monitoring in Ukraine
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2015, Vol 7, Issue 1
Abstract
The ELISA screening of beet necrotic yellow vein virus (BNYVV) in agrocenoses of Ukraine (Western and Central Ukraine) was performed and the presence of BNYVV was shown in samples of roots of sugar beet plants selected in Chemerovetsky region of Khmelnitsky oblast. The bioinformative analysis of nucleotide sequences of isolates of BNYVV deposited in genetic databank was made. The conserved gene sequence encoding coat protein was established and specific primers for nucleotide sequences of the genome of the BNYVV were developed. The specific oligonucleotide primers were synthesized. The laboratory testing of synthesized primers and the optimization of polymerase chain reaction were made. The optimum temperature of primers hybridization was 54 °C - 62 °C and the highest production of amplicons was registered by the concentration of Mg+2 was 2,5 mM.
Authors and Affiliations
К. Гринчук, І. Антіпов, М. Єрмолаєв
Keywords
Related Articles
- EP ID EP236445
- DOI -
- Views 84
- Downloads 0
Mires of the magnocaricion elatae w. Koch 1926 s. L. in Ukrainian Carpathians and adjacent areas
The article summarizes the data on mires of the Magnocaricion elatae s.l. from Ukrainian Carpathians (incluiding Ciscarpathian and Transcarpatian plains). 66 relevés are provided. They are grouped into 7 associations: Ca...
5S rDNA of timothy- grass Phleum pratense L.
Genomic regions encoding 5S ribosomal RNA (5S rDNA) are necessary for ribosomal biogenesis. Tandemly arranged repeated units of 5S rDNA found in the genomes of all eukaryotes represent a universal model for studying the...
Optimisation of ecologically safe and effective use of arable lands in chernivtsi region
Definition of the essence and significance of zoning of land resources for the organization of rational and ecologically safe land use have been presented. The placement of Chernivtsi region in the national system of sin...
Changes of state and structure of Schivereckia podoliсa (Besser) Andrz. ex DC. (Brassicaceae) coenopopulations in the conditions of original habitat types of the Smotrich canyon (Кmyanets-Podilsky city, Khmelnytsk region) for 10 years
Results of study of coentopopulations age structure changes of Schivereckia podolica (Besser) Andrz. ex DC. (Brassicaceae) local population in the conditions of the Smotrych Canyon on the territory of Kamenets-Podilsky c...
Genus Rubus L. (Rosaceae Juss.) in the flora of Western Ukraine: 4. Subgenus Rubus sections Corylifolii and Caesii, subgenera Idaeobatus and Cylactis
The genus Rubus L. is critical and problematic for taxonomy. In the west of Ukraine conducted only primary inventory of Blackberry diversity and information about species distribution is fragmentary. The results of flori...