THE DR FAMILY - AFIMBRIAL ADHESIN GENE IN UROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM PATIENTS SUSPECTED WITH URINARY TRACT INFECTION.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2019, Vol 7, Issue 3
Abstract
Background: UropathogenicEscherichia coli (UPEC), possess different adhesin factors for binding the cells of urinary tract, afimbrialadhesin coded by the gene afa is one among the adhesin factors belonging to Dradhesin family. afa facilitates the colonization by binding to the receptor on the Dr blood group antigen, it stimulates the cytopathic effect and various signal cascade reactions. This adhesin accounts for chronic and recurrence in UTI. Aim & Objective: The aim of the study is to determine the prevalence of afa gene by using molecular method PCR. Materials & Methods: Bacteria isolated from urine samples were processed and confirmed for E. coli using standard bacteriological methods. DNA extraction done followed by PCR amplication were processed for detection of afa genes using specific primer and the specific base pair bands were noted using gel documentation. Results:The afa gene was found in 49 isolates (23.1%) of UPEC out of 212 isolates, which were distributed among male patients 19(38.8%) and 30 (61.2%) in female patients with more prevalence among less than 20 years of age group. Conclusion:afa, though a low frequency gene in UPEC showed high percentage in this study comparing the other geographical areas out of its low frequency.
Authors and Affiliations
SM. Nachammai, Karthika Jayakumar, Vinithra Suresh, Kousalya M.
Keywords
Related Articles
- EP ID EP535903
- DOI 10.21474/IJAR01/8622
- Views 52
- Downloads 0
AN EPIDEMIOLOGICAL ASSESSMENT OF CHILD INJURIES IN ZAGAZIG UNIVERSITY HOSPITALS; PATTERNS AND MANAGEMENT MODALITIES.
Background: Trauma is the leading cause of death in childhood. Among children aged from 1 to 14 years, approximately 50% of mortality is related to trauma. Aim: to evaluate child injury in Zagazig University Hospitals in...
COMPARATION OF THEORETICAL PROPERTIES OF 3-METHYL-4-(3-BENZOXY-4-METHOXYBENZYLIDENAMINO)-4, 5-DIHYDRO-1H-1, 2, 4-TRIAZOL-5-ONE MOLECULE.
3-Methyl-4-(3-benzoxy-4-methoxybenzylidenamino)-4,5-dihydro-1H-1,2,4-triazol-5-one molecule was optimized by using the B3LYP/HF 631G(d,p) and B3LYP/HF 6311G(d,p) basis sets. This optimized structures used to calculation...
EXTREMITY VASCULAR INJURIES ? A PROSPECTIVE STUDY.
Introduction: Extremity vascular trauma is common in civilian population following RTA. The aim of this study is to evaluate the results following intervention in extremity vascular trauma cases admitted in Rajiv Gandhi...
SENSORY IMPROVEMENT AND REDUCTION OF NERVE THICKENING IN A LEPROSY PATIENT TREATED WITH ULTRASOUND GUIDED PERINEURAL INJECTION OF AUTOLOGOUS PLATELETRICH PLASMA: A CASE REPORT AND REVIEW OF LITERATURE.
Background: Leprosy is caused by Mycobacterium leprae which has a certain predilection for peripheral nerves. It causes peripheral neuropathy and in the long run, it causes deformity of hands and feet. Platelet-rich plas...
EFFECT OF INJECTION TIMING AND NOZZLE HOLE GEOMETRY ON COMPRESSION IGNITION ENGINE FUELLED WITH THEVETIA PERUVIANA BIODIESEL.
In the present study an experimental work had been carried out to analyze the performance and emission characteristics of various blends of thevetiaperuviana biodiesel by varying injection timings and nozzle hole geometr...