The effect of ascorbic acid on hatching performance and tolerance against environmental stressor (high temperature) by immersion of Prussian carp (Carassius gibelio) fertilized eggs
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2010, Vol 3, Issue 3
Abstract
The aim of this study was to evaluate the influence of L-ascorbic acid (AA) in three levels (0,100, 1000 and 2000 mg L-1) on eyed egg and hatching rate, growth and viability of larva, and larval tolerance against high temperature stress of Prussian carp Carassius gibelio (Bloch, 1782). The fertilized eggs were placed in water containing different levels of AA for 3 h. The percentage of eyed egg and hatching were measured after 2 and 3 days respectively. After larva absorbed their yolk sac, half of them were challenged by high temperature (30°C) and the others were reared for 45 days and growth factors and survival were calculated. The result shown that the highest eyed egg and hatching rate were in 2000 mg L-1 and had significantly differece with other treatments (P<0.05). The significant differences in larval tolerance against high temperature stress were observed in 1000 and 2000 mg L-1 compared to 0 and 100 mg L-1 treatments. No significant differences were observed between growth parameters of treatment batches (P>0.05). Viability was different between experimental groups, but it was not significant between 0 and 100 mg L-1. According to our results when broodstocks of Prussian carp do not have enough vitamin C in their ovaries, immersion of fertilized eggs in 2000 mg L-1 of AA may be beneficial.
Authors and Affiliations
Mohammad Taati, Bahareh Mehrad, Ali Shabani
Keywords
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