The Effect of Cocoa Pod Husk (Theobroma cacao L.) Extract on Increasing Gingival Fibroblast and Collagen Density in Wistar Rats Induced by Porphyromonas gingivalis
Journal Title: International Journal of Multidisciplinary Research and Analysis - Year 2024, Vol 7, Issue 02
Abstract
Periodontal disease is an inflammatory disease of the oral cavity which characterized by inflammation of the gingiva. Theobroma cacao L. is a herbal plant that has antibacterial and anti-inflammatory effects, which is starting to be widely researched as an alternative therapy for inflammation in the oral cavity. The aim of this study was to examined the effect of the ethanol extract gel of cocoa pod husk (Theobroma cacao L.) on increasing the number of fibroblasts and the collagen fiber density in the gingiva of rats that have been induced by Phorphyromonas gingivalis. The sample used 24 male Wistar rats divided into 3 groups. Each group was induced by porphyromonas gingivalis every 3 days for 14 days then anointed with placebo gel (negative control group), metronidazole gel (positive control group), and cocoa pod ethanol extract gel (CPH gel) for 7 and 14 days. Gingival tissue was taken after euthanasia, after which histological preparations were made, and fibroblasts were observed under a microscope and analyzed using quantitative pathology 0.3.2 software, while for collagen density analized using Adobe Photoshop CS6 software. The results showed there was a significant difference of the number of fibroblasts between each group (p<0.05). however there was no significant difference between positive control and cocoa pod husk extract at day 14 (p > 0.05).The treatment group on day 7 and 14 was higher than the other groups. It was conclude that the ethanol extract of cacao pod husk (Theobroma cacao L.) potential to increase the number of fibroblasts and collagen fibers density in gingival tissue of periodontitis rats.
Authors and Affiliations
Sukanto, Yani Corvianindya Rahayu, Herniyati, Happy Harmono, Raikhan Mahadi
Keywords
Related Articles
- EP ID EP731481
- DOI 10.47191/ijmra/v7-i02-47
- Views 108
- Downloads 0
Do we Need Prophylactic Salphingectomy for Ovary Preserving in
Ovarian carcinoma was believed to had arised from fallopian tubes and then spread to ovarian surface and peritoneum. In addition, Lack of ovarian lesion precursor identified and the fact that bilateral salphingo-Oophere...
Giant Floater (Pyganodon Grandis) as Feed Ingredient in Broiler Production
The focus of the research was to determine how giant floater (Pyganodon grandis) worked as a feed ingredient in broiler production. A total of 120 one-day-old Cobbs 500 were reared for 42 days, regardless of sex. The chi...
Commodity Analysis of Carbohydrate Food Availability in the Banyumas Regency Area
The study aims to determine the potential of carbohydrate food sources regarding the availability of carbohydrate food sources in Banyumas Regency. The research method is carried out through descriptive research. The dat...
The Influence of Leadership Style, Individual Characteristics and Work Environment on Member Performance at Kesdam XII/Tanjungpura Pontianak
The purpose of this study was to analyze the influence of leadership style, individual characteristics and work environment on the performance of members in Kesdam XII / Tanjungpura Pontianak, either simultaneously or pa...
Third-Person Passive Voice in Illustrative Explanatory Speech Acts by Residents of Sumberejo, Ambulu, Jember
Third-person passive voice as one of the many topics of grammatical semantics is used to describe the relationship between the third actor and the action in the form of passive verbs in a grammatically meaningful sentenc...