THE EFFECT OF HEAT STRESS ON HYPERHYDRICITY AND GUAIACOL PEROXIDASE ACTIVITY (GPOX) AT THE FOLIAR LAMINA OF SEDUM TELEPHIUM L. SSP. MAXIMUM (L.) KROCK. VITROPLANTLETS
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2014, Vol 60, Issue 2
Abstract
Heat stress causes an array of physiological, biochemical and morphological changes in plants, affecting plant growth and development. In order to confirm the connection between oxidative stress in plants and hyperhydricity (vitrification), we used a Sedum telephium ssp. maximum plant as a test material. The changes of the antioxidant enzyme guaiacol peroxidase (GPOX) activity in in vitro Sedum plantlets were studied under increased temperatures and compared with in vitro plantlets kept at 21ºC as the control. The results showed that the highest activity of GPOX was in response to heat stress of 41°C on day 10 after treatment (102,32 UI/mL), compared with the control where the GPOX activity remained unchanged (38,1 UI/mL) until the end of the experiment (40 days). In case of plants subjected to heat stress starting from the first days of vitroculture, the vitrification process has begun and with increasing of GPOX activity, vitrification was becoming more intense. However, the vitroplantlets grown for 40 days under constant temperature showed no signs of vitrification. Cytological studies of the hyperhydric foliar lamina were also performed at the end of 40 days of the experiment. Microscopic studies have shown that the hyperhydric mesophyll cells of S. telephium ssp. maximum are in a process of necrosis, disorganization, extremely low on cytoplasm, have an imploded cellular content and big gaps between them. The results from this study suggest that the heat stress plays a role in triggering the vitrification process in the in vitro micropropagated plants.
Authors and Affiliations
MIRELA ARDELEAN, DORINA CACHIŢĂ-COSMA, AUREL ARDELEAN, CALIN LADAŞIU, VALENTIN MIHALI
Keywords
Related Articles
- EP ID EP163333
- DOI -
- Views 90
- Downloads 0
ALGOFLORA FROM THE MILETIN AND JIJIA INFERIOR CATCHMENTS II. PHYLUMS CHLOROPHYTA, HETEROKONTOPHYTA, CHOANOZOA AND CRYPTOPHYTA
The paper presents a continuation of the detailed analysis of the algal flora from the Miletin and Jijia Rivers inferior catchments located in the proximity of Iaşi city and of a series of reservoirs complexes created on...
EFFECT OF LEAD ON SEED GERMINATION AND SEEDLING DEVELOPMENT IN SOME MEDICINAL PLANTS (1st note)
The tolerance of lead in two medicinal and aromatic plants (Ocimum basilicum L. and Salvia officinalis L.) was investigated using seed germination and plant growth bioassays.
A NEW SITE IN ROMANIA FOR SPIRULINA (ARTHROSPIRA) PLATENSIS
Spirulina (Arthrospira) platensis Geitl was found in the perimeter of the Tomesti village (district Iasi) for the first time in Moldavia (Romania). Its coenotic ambience and distribution in Romania are given.
CHARACTERISTICS OF THE PLANKTONIC ALGOFLORE FROM DOROBANTI, ARONEANU, CIRIC I, II AND III LAKES (IASI COUNTY)
After the setting of Ciric river by building some barrages, the lakes Dorobanti, Aroneanu, Ciric I, II and III were formed, their total surface being of about 123 ha. When the lakes were formed, processes of colmat...
THE ECOLOGICAL CHARACTERIZATION OF THE FORESTRY ASSOCIATIONS FROM THE MIDDLE STREAM OF THE NIRAJ VALLEY (ROMANIA, MUREŞ COUNTY)
The aim of this study was the qualitative structure assessment of the forestry associations sustaining a large number of rare or declining bird species, reason that the Special Protection Area was established. These are...