The effect of metals nanoparticles on activation of metabolic processes in cells C. Perfringens type A
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. One of the areas of metal nanoparticles application in biotechnology of vaccines manufacturing is their use in technological schemes as catalysts of metabolic processes in pathogens strains cells to obtain large amounts of bacterial mass. The goal of the work was to determine the smallest stimulating concentration of gold (AuNP), silver (AgNP), copper (SuNP) and iron (FeNP) nanoparticles contributing to the accumulation of large amounts of C. perfringens type A bacterial mass. Materials and methods. The work was performed in the V.Ryzhenko laboratory of anaerobic infections. AuNP, AgNP, SuNP, and FeNP were synthesized at the F.D. Ovcharenko Institute of Biocolloidal Chemistry of the NAS of Ukraine. To account the quantitative content of bacterial cells C. perfringens type A after culture in the presence of various concentrations of metal nanoparticles research. Accounting of the quantitative content of C. perfringens type A bacterial cells was conducted after cultivation in the presence of various concentrations of metal nanoparticles.Results of research and discussion. Cultivation of C. perfringens type A in the presence of AuNP in 0.965 μg/ml concentration for 36 hours facilitated significant increase of bacterial mass by growth coefficient in 2.06 times increase (p> 0.01) as compared with the initial data. The results of studies on the influence of AgNP on C. perfringens type A cells have shown that bacterial growth in the presence of 20.0 mcg/ml of the nanopreparation within 24 hours contributed to the increase of bacterial mass volume by growth coefficient in 4.02 times (p> 0.001) compared to initial data. The influence of СuNP in 0.040 mg/ml concentration on the pathogen growth stimulation showed increase of bacterial mass in 24 hours in 3.92 times (p> 0.001) by growth coefficient compared to the data in the beginning of the experiment. The optimal stimulating concentration of FeNP is determined to be 0.310 mg/ml as cultivation within 24 hours significantly increased bacterial mass of this pathogen in 23.5 times (р>0,001) compared to initial data. Conclusions and prospects for further researches. The optimal stimulating concentrations of nanoparticles have been determined: AuNP - 0.965 mg/ml, AgNP - 10.0 mg/ml, CuNP - 0.040 mg/ml and FeNP - 0.625 mg/ml in the presence of which cultivation of Cl. perfringens type A for 24 hours provides obtaining significant volume of bacterial mass.Prospects for further research are the development and testing of vaccines containing nanoparticles of metal against bacterial infections of animals.
Authors and Affiliations
Olga Gorbatyuk, Valentina Andriyaschuk, Galina Ryzhenko, Alexander Zhovnir, Lyudmila Reznichenko, Svetlana Dybkova
Keywords
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- EP ID EP267275
- DOI 10.31073/vet_biotech30-05
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