The Effect of Pare Ethanol Extract on Bax and Bcl-2 Protein Expressions (In vitro study on T47D Breast Cancer Cell Culture)
Journal Title: International Journal of Multidisciplinary Research and Analysis - Year 2023, Vol 6, Issue 03
Abstract
Breast cancer is a genetic disease due to the accumulation of genetic abnormalities in tissues. There are cell proteins that can reduce the growth of cancer cells. Bax and Bcl-2 are proteins that accelerate the process of apoptosis of cancer cells and maintain the permeability of mitochondrial cell membranes and cytotoxic activation of caspase so as to prevent the spread and development of cancer cells. The purpose of this study was to determine the effect of pare ethanol extract on the expression of Bax and Bcl-2 in T47D cell cultures. This research uses experimental in vitro with the Post Test Only Control Group Design method. The subjects of this study were T47D cells divided into 4 treatments with three replications on the variables. The treatment consisted of negative control, positive control, pare ethanol extract 1/2 IC50 (134.7 g/mL), 1 IC50 (269.4 g/mL) and 2 IC50 (538.8 g/mL). On the 10th day, an examination of Bax and Bcl-2 expressions was carried out. The data were analyzed using the Anova One Way Test. One Way Anova test result showed the results of Bax protein expression and BCL-2 expression in all groups there was a significant difference (p< .05). The administration of pare ethanol extract at dose of 2 IC50, 1 IC50, and 1/2 IC50 showed a significantly affect to increase of Bax protein expression in T47D breast cancer cells.
Authors and Affiliations
Dewi Anggrahenny, Titiek Sumarawati, Chodidjah
Keywords
Related Articles
- EP ID EP714140
- DOI 10.47191/ijmra/v6-i3-25
- Views 63
- Downloads 0
Strategic Management to Improve Service Quality and Customer Satisfaction in Fitness Centres: A Multi-Case Study of Two Types of Fitness Centres in Bogor
This multi-case study aims to analyse the strategic management picture to improve service quality and customer satisfaction of two types of Bogor Fitness Centres. The method used in this research is mixed methods. The co...
Proposed Web-Based Goods Recording Information System in the Warehouse of PT Bahana Unindo Teknik Plant Plastics Injection
PT Bahana Unindo Teknik Plant Plastics Injection is a company engaged in manufacturing. In the company's warehouse there is a problem, namely the absence of an integrated goods recording system, this is an obstacle becau...
Hydrochemical and Bacteriological Studies of Streams in Calabar South Local Government Area, Nigeria
This paper presents the results of hydro-chemical and bacteriological analysis of water obtained from streams in Calabar South Local Government Area in Nigeria. Water samples were collected from five different streams in...
Cost Efficiency Through the use of the EOQ Method
This study aims to analyze and show how to calculate EOQ and prove that the use of the EOQ method can make inventory costs efficient because the number of purchases is not excessive and not lacking, the frequency of purc...
On the Identity Construction and Industrial Development of "Digital Self-Maker" In Online Sales in the Digital Age
With the development of network and digital technology, modern business began to turn to online sales. Relying on digital technology, database technology and Internet of Things (IoT) technology, virtual online store has...