The effect of six common PCR inhibitors on DNA polymerase and DNA template
Journal Title: International Journal of Forensic Science & Pathology (IJFP) - Year 2014, Vol 2, Issue 8
Abstract
In order to elucidate the effect of PCR inhibitors on DNA polymerase and DNA template, two main components of the PCR reaction, in this paper, we selected six representative PCR inhibitors, bile salts, collagen, humic acid, hemoglobin, melanin and urea, and added respectively them to the K562 DNA with the standard addition concentration in which all of 26 different alleles of the K562 DNA with the AmpFℓSTR® Identifiler® PCR Amplification Kit are just inhibited completely. On this basis, after increased the amount of K562 DNA and DNA polymerase respectively, PCR amplification and STR genotyping were performed, and then counted the allele number of K562 DNA detected. When the DNA polymerase was increased to a certain amount, all of 26 alleles of the K562 DNA mixed with various PCR inhibitors could be detected successfully, and when the amount of K562 DNA was increased, the inhibitory effect of bile salts, collagen and urea had only been eliminated partly and no significant improvement was observed for the other three inhibitors. The results indicate that all of the six representative PCR inhibitors mainly inhibit DNA polymerase, and increasing the amount of DNA polymerase in PCR reaction can improve significantly the efficiency of PCR amplification and the success rate of genotyping.
Authors and Affiliations
Daixin Huang
Keywords
Related Articles
- EP ID EP200341
- DOI 10.19070/2332- 287X-1400017
- Views 116
- Downloads 0
Correlation of Troponin T Levels in the Cardiac Sudden Death Cases at Hospital Kuala Lumpur
Cardiac deaths accounted for 50% of all deaths in the developed and one fourth in the developing world. Sudden cardiac death due to an acute myocardial infarction (AMI) comprises a significant proportion of autopsy cases...
Forensic scientific evidence: problems and pitfalls in India
Forensic science, the sibling of science has transformed the criminal adjudication especially the criminal trial process. There is no doubt about the role of forensic science in helping the judiciary in settling issues w...
Cheiloscopy - A Vital Tool In Crime Investigation
Background: Various methods are used to collect evidence from a crime scene, and with the advancements in forensic science, most techniques are a common and necessary part of all criminal investigation. Cheiloscopy or li...
Role Of Diatoms In Forensic Investigation: Case Studies From Haryana
Diatoms, the most common type of phytoplankton, are a major group of eukaryotic algae which are ubiquitous to a wide variety of aquatic habitats. These are useful in linking suspects and victims to crime scenes in and ar...
Sudden Unexpected Death Due To Glottic Oedema – Report Of Two Cases
The space between the vocal cords and the mucosa covering them is the glottis. Glottic oedema can be caused by various reasons like hypersensitivity, inflammation, infection or by mechanical obstruction. Glottic oedema c...