The effects of different concentrations of NanoSilver on elimination of Bacterial contaminations and phenolic exudation of Rose (Rosa hybrida L.) in vitro culture
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 1
Abstract
Rose is known as the first and the most important cutflowers in all over the world. One of the common methods of mass production of this plant is propagation through tissue culture. The main limiting factor of rose tissue culture is Bacterial contaminations and phenolic exudation in the establishment phase that significantly makes most of explants spoiled. Nano-Silver is able to control and stop the bacterial contamination. In this experiment Nano-Silver was added to medium with concentrations of ( , , and ppm) and in the other experiments explants were immersed in solutions of Nano-Silver with concentrations ( , , and ppm) in complete random design. The experiments were carried out with four replications. The results showed that the concentration of ppm which is directly added to the medium can reduce Bacterial contamination and phenolic exudation rate. Concentration of ppm for minutes after surface sterilization was the best treatment of immersion to control bacterial contaminations. High concentrations of Nano-Silver make regeneration of explants more and more slower and in some cases lead to destroy explants. In general, Nano-Silver had no effects on fungal contamination.
Authors and Affiliations
Saber Shokri, Ali reza Babaei, Marzieh Ahmadian, Shahriar Hessami and Mohammad Mehdi Arab
Keywords
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