The effects of ozone on the local and systemic interleukin 1β (IL-1β) and IL-10 levels experimental periodontitis model in rats
Journal Title: Cumhuriyet Medical Journal - Year 2017, Vol 39, Issue 3
Abstract
Objective: The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to the administration of ozone. Method: Twenty-four rats were randomly divided into three experimental groups: a nonligated (NL) treatment group (n = 8), a ligature-only (LO) treatment group (n = 8), and a ligature plus ozone (60 second/each tooth a day) (LOZ) treatment group. In order to induce experimental periodontitis, a 4/0 silk suture was placed at the gingival margin of the right-mandibular first molars of the rats. The study duration was 14 days, and then the animals were sacrified. Changes in the alveolar bone levels of rats in each group were measured clinically, and the tissues of the rats in each group were examined histopathologically to determine inflammatory cell infiltration (ICI), osteoblast and osteoclast activities, and osteoclast morphology. Serum and gingival cytokine levels were measured by using the rat-specific IL-1β and IL-10 ELISA kits. Results: Alveolar bone loss around the mandibular molar tooth was significantly higher in the LO group compared with NL and LOZ groups (p<0.05). The ratio of the presence of ICI and osteoclast numbers were significantly higher in the LO group than in the NL and LOZ groups (p<0.05). Osteoblastic activity was significantly lower in the LO group than in the NL and LOZ groups (p<0.05). The serum and gingival homogenate IL-1β levels in the LO group were statistically higher than in the NL and LOZ groups (p<0.05). The serum IL-10 level in the LO group was statistically lower than in the NL and LOZ groups (p<0.05). The gingival homogenate IL-10 level in NL group was statistically higher than in the LO group (p<0.05). Conclusions: The present study showed that the oral administration of ozone diminishes alveolar bone resorption in a rat periodontitis model.
Authors and Affiliations
Aysun Akpınar, Metin Çalışır, Ömer Poyraz, Fahrettin Göze, Derya Özdemir Doğan, Vildan Bostancı
Work-related muscoskeletal symptoms and work impairment among workers in Turkish companies
Objective: Musculoskeletal disorders are major problems for the working population. The aims of study were to describe musculoskeletal symptoms (MSS) and work impairment among workers and to analyze the relationships bet...
Acute administration of high dose trimetazidine inhibits pentylenetetrazol-induced seizures in rats
Objective: Resistant epilepsy is widely seen in clinical settings manipulate the researchers to pursuit novel anti-epileptic treatments. Although trimetazidine (TMZ) is commonly used in the treatment of angina and ischem...
Researching the effect of the practical applications performed with cadaver dissection and anatomical models on anatomy education
Objective: The most important element in providing good quality of healthcare is well training of healthcare staff, particularly doctors. The way of being a good and successful healthcare staff is to learn the human anat...
Location, sidedness, and sex distribution of incidental intracranial arachnoid cysts in childhood: An MRI study
Objective: The objective of this study was to evaluate retrospectively the MRI features of incidental intracranial arachnoid cysts with regard to their distribution of cyst location, sidedness, Galassi type, and gender i...
Evaluation of abdominal aortic aneurysm rupture in emergency department: Case report
Abdominal aortic aneurysm (AAA) is a common chronic degenerative disease of the abdominal aorta leading to progressive dilatation, thrombosis, rupture and distal embolization. It is defined as exceed 30 mm or 50% increas...