The establishment of a high-resolution melting curve genotyping method for ABCG2*376T allele of Jr(a-) and its distribution frequency study
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 7
Abstract
Objective To establish a high-throughput detection method for ABCG2*376T allele of Jr(a-), and apply it to the study of the frequency of this allele in the Chinese population. Methods The specific primers were designed and synthesized, the sample carrying homozygous ABCG2*376T alleles, obtained in the previous study, was used as the homozygous positive control, and the sample carrying heterozygous allele as the heterozygous positive control. The wild-type sample was used as a negative control, and a high-resolution melting curve(HRM) method for detecting this allele was established. The established method was used to screen DNA samples from blood donors in Guangzhou, and the samples carrying ABCG2*376T alleles were sequenced to confirm the accuracy of the HRM method. Results A HRM method, which can detect ABCG2*376T allele and accurately type homozygotes and heterozygotes at the same time, had been established successfully. Fifteen individuals with heterozygous alleles were screened out of 1 560 blood donors in Guangzhou, while none homozygous allele was detected. Conclusion The HRM method can be used to accurately screen and type ABCG2*376T allele. The frequency of this allele in Chinese population is about 0.48%(15/3120).
Authors and Affiliations
Zhen WANG, Shuangshuang JIA, Zhijian LIAO, Chunyan MO, Ling WEI, Runqing ZHANG, Guangpin LUO, Yanli JI
Keywords
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- EP ID EP738282
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.07.002
- Views 43
- Downloads 0
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