THE FEATURES OF BIOCHEMICAL PARAMETERS CHANGE IN DIFFERENT ECOLOGICAL GROUPS OF FISH UNDER CONDITIONS OF ANTHROPOGENIC PRESSURE
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
An influence of environmental conditions on the content of hormone thyroxine, triiodothyronine, LDH activity and glucose in the blood plasma of bleak, roach, rudd, ruff and river perch was considered. The content of hormones thyroxine and triiodothyronine was determined by ELISA using standard commercial kits. Glucose was determined by glucosoxidase method using standard sets on a spectrophotometer SF-26. Activity of lactate dehydrogenase was assessed spectrophotometrically at the wavelength of 345 nm using SF-26. The changes in the content of triiodothyronine in the blood plasma of the studied species were considered. It was found that the content of this hormone was lower in perch, ruffe, gossip and scardinius which were lived in the anthropogenically disturbed Cyrilovske Lake than in fish from the Bila Tserkva medium reservoir (Ros River). It indicates a decrease in activity of redox processes. It was established that in all investigated fishes from the Cyrilovske Lake the lactate dehydrogenase activity was increased. It is indicated on widely involvement of glycolysis in adaptive processes. The opposite reaction has been seen in bleak. In contrast to the above mentioned species the content of triiodothyronine was higher in fish from Cyrilovske Lake in comparison with reservoir. It is explained by interspecies differences in metabolism and the fish habitats in the pond. It is noted that an increased glucose level in the blood plasma is connected with increasing of LDH activity. However, it is common only for perch, ruffe and roach, which obviously indicates on a significant need for energy supply of the studied fish. Based on the research data the revealed interspecific difference of changing the content of certain hormones and the activity of LDH and glucose was demonstrated. The investigated parameters can be further used for biomonitoring and identify water-resistant and vulnerable species.
Authors and Affiliations
М. В. ПРИЧЕПА, О. С. ПОТРОХОВ, О. Г. ЗІНЬКОВСЬКИЙ
Keywords
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