The influence of shielded magnetic field on the cell viability and nitrogenase activity of Azotobacter chroococcum.
Journal Title: Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society - Year 2009, Vol 1, Issue 1
Abstract
This paper studies the magnetic field influence on the viability of zotobacter chroococcum 2286T cells. The viability of the Azotobacter chroococcum cells incubated under shielded magnetic field was established according to the nitrogen acumulation rate in the culture medium, related to the control samples incubated in normal lab conditions. The inoculation was made from solid medium, and from liquid medium, at an bacterial density of 1.5∙106. After incubation the NH[sub]4[/sub][sup]+[/sup], NO[sub]2[/sub][sup]-[/sup] and NO[sub]3[/sub][sup]-[/sup] ions concentration in the medium was determined. The total nitrogen quantity was determined through Kjeldahl method. The nitrogenase activity was established by the reduction of C[sub]2[/sub]H[sub]2[/sub] to C[sub]2[/sub]H[sub]4[/sub] and by detecting it using a gas chromatograph.
Authors and Affiliations
Cristina Dobrotă, Rahela Carpa
Keywords
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