The Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N Mice Strain on Escherichia coli LF82 Invasion
Journal Title: Bezmiâlem Science - Year 2019, Vol 7, Issue 3
Abstract
Objective: The aim of this study was to investigate the effect of healthy mucosa on adhesive and invasive properties of AIEC reference strain Escherichia coli (E. coli) LF82. For this purpose, we had designed special medias that contained cell culture medium and mucosal content obtained from different regions (colon and ileum) of the digestive tract. Methods: We tested the infecting ability of AIEC reference strain E. coli LF82 on I-407 cells in the presence of mucosal media (Muc-M) under in vitro conditions. Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cellinfection experiments and biofilm-formation assays. Results: The result showed that the mucosal media decreased the infection percentage of E. coli LF82 strain when compared with control group. It was seen that the mucosal media originating from ileum almost completely inhibited the invasion of LF82 strain. On the other hand, it was observed that the mucosal media prepared from colon) reduced the bacterial invasion only in half the rate when compared with control. Conclusion: The findings showed that these medias obtained from different regions of the intestinal tract affected LF82 invasion at different rates. Therefore, this study provided crucial information that could contribute to the future studies on the localization of bacteria.
Authors and Affiliations
Hüsamettin AYGÜN, Murat KARAMEŞE, Fikret UYAR
Keywords
Related Articles
- EP ID EP50746
- DOI 10.14235/bas.galenos.2018.2627
- Views 224
- Downloads 0
Prolonged Mechanical Ventilation Predictors in Patients Undergoing Liver Transplantation
Objective: Liver transplantation is the current treatment modality for end-stage liver disease. While there is increasing drive towards early extubation in patients undergoing liver transplantation, prolonged meachanical...
Effect of Flavopiridol on Cell Cycle, Apoptosis and Biomolecule Structure Changes in Breast Cancer Stem Cells
Objective: Cancer stem cells (CSCs) are a small population in cancer, which are responsible for therapeutic resistance, relapse and metastasis. Flavopiridol has antitumor activity against various types of cancer cells. T...
The Relation of The Duration of Work in Dentists with Postural Problems, Range of Motion and Pain
Objective: The aim of this study is to determine the relationship between cervical and lumbar range of motions, pain and postural problems with the time spent in the occupation in dentists. Methods: A total of 56 dentis...
Nutritional Assessment and Physical Activity of the Departments for Nutrition&Dietetics and Nursing Students at a Foundation University
Objective: Nutritional assessment and physical activity habits of nutrition & dietetics students (experimental group) who took a course of The Principles of Nutrition and nursing students (control group) who did not take...
Evaluation of The Effects of Raisins and Hazelnuts Added To the Diet on Lipid Profiles and Anthropometric Measurements in Women with Hyperlipidemia
Objective: The positive effects of nuts and grape products on lipid profiles have been proved by epidemiological and clinical studies. However, studies investigating the effect of raisins on lipid profiles are limited. T...