The p53 Mutation/Deletion Profle in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma
Journal Title: Sultan Qaboos University Medical Journal - Year 2014, Vol 14, Issue 1
Abstract
Objectives: Mutations/deletions affecting the TP53 gene are considered an independent marker predicting a poor prognosis for patients with diffuse large B-cell lymphoma (DLBCL). A cohort within a genetically isolated population was investigated for p53 mutation/deletion status. Methods: Deoxyribonucleic acid (DNA) samples were extracted from 23 parafn-embedded blocks obtained from DLBCL patients, and subjected to polymerase chain reaction (PCR) amplifcation and sequencing of exons 4–9 of the p53 gene. Results: While 35% of patients analysed displayed allelic deletions (P <0.01), immunohistochemical analysis revealed a mutation rate of 69.5%. It is noteworthy that the rate of p53 mutations/deletions in this small cohort was found to be higher than that previously reported in the literature. Interestingly, patients with p53 mutations displayed a better overall survival when compared to those without. Te survival of patients treated with rituximab-containing combination chemotherapy was signifcantly better than those who did not receive rituximab (P <0.05). Furthermore, a modelling analysis of the deleted form of p53 revealed a huge structural change affecting the DNA-binding domain. Conclusion: Te TP53 mutation/deletion status plays a role in mechanism(s) ruling the pathogenesis of DLBCL and may be useful for stratifying patients into distinct prognostic subsets.
Authors and Affiliations
Yahya Tamimi| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Sheikha Al-Harthy| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Ibrahim Al-Haddabi| Deapartment of Pathology, Sultan Qaboos University Hospital, Muscat, Oman, Mohammed Al-Kindi| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Hamza Babiker| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Mansour Al-Moundhri| Departments of Medicine, College of Medicine & Health Sciences, Sultan Qaboos University, Ikram Burney| Departments of Medicine, Sultan Qaboos University Hospital, Muscat, Oman
18F-Fluorodeoxyglucose-Positron Emission Tomography/Computed Tomography in the Management of Metastatic Colorectal Cancer Are we there yet?
Emergence of Diseases of Affluence in Oman Where do they Feature in the Health Research Agenda?
THE HEALTH STATUS AND LIVING conditions in emerging economies, sometimes called developing countries, have changed considerably because of the rapid growth of the biomedical healthcare infrastructure and acculturation an...
Multiple Bone Metastases in a Patient with Differentiated Thyroid Cancer (DTC) Complete resolution following thyroidectomy and four ablation doses of I-131
Multiple bone metastases from a differentiated thyroid cancer are usually incurable. We report the case of a young Omani woman who presented with 8 discrete skeletal lesions three years after a total thyroidectomy. Fo...
Aetiologies of Hyperprolactinaemia: A retrospective analysis from a tertiary healthcare centre
ABSTRACT: Objectives: This study aimed to evaluate the aetiologies of hyperprolactinaemia in the United Arab Emirates (UAE). Methods: This retrospective study used laboratory databases to identify all patients who underw...
Re: Silent Epidemic of Depression in Women in the Middle East and North Africa Region