The Protective Effects and Mechanism of Mulberry Leaf Flavonoids on Type 2 Diabetic Nephropathy Mice
Journal Title: Journal of Experimental and Clinical Application of Chinese Medicine - Year 2024, Vol 5, Issue 4
Abstract
Purpose: This study investigated the protective effects and mechanism of mulberry leaf flavonoids on type 2 diabetic nephropathy (DN) in mice. Methods: The DPPH free radical scavenging activity of mulberry leaf flavonoids was measured in vitro. To establish a type 2 DN model, male ICR mice were induced with streptozotocin (STZ) after a high-fat diet regimen. The mice were then divided into groups: a normal control group, a DN model group (DM), a positive control group receiving 200 mg/kg metformin (Met group), and a treatment group receiving 1 g/kg mulberry leaf flavonoids (FM group). Both Met and FM groups received daily intragastric administration for 4 weeks, while the DM and control groups were given equivalent volumes of normal saline. Fasting blood glucose levels were recorded regularly during the intervention. After the treatment period, serum creatinine (CRE), blood urea nitrogen (BUN), superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, and renal glutathione peroxidase (GPx) activity were measured. Additionally, kidney histopathological changes and glycogen deposition were evaluated by HE and PAS staining, while Nrf2 and HO-1 expression in kidney tissue was assessed through immunohistochemistry. Results: Mulberry leaf flavonoids effectively scavenged free radicals, significantly reduced blood glucose levels, CRE, BUN, and MDA in serum and kidney tissues, and increased SOD and GPx activities. Histopathological improvements in kidney and pancreatic tissues were observed, alongside increased Nrf2 and HO-1 expression. Conclusion: Mulberry leaf flavonoids ameliorate renal damage in DN mice, potentially through oxidative stress reduction and activation of the Nrf2/HO-1 signaling pathway.
Authors and Affiliations
Huajie Hu, Yanna Guo, Leifang Zhang, Ke Li
Keywords
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