THE STUDY OF THE POLYMORPHISM OF LOCUS BMP 15 OF THE SHEEP IN THE SOUTHERN REGION OF UKRAINE
Journal Title: Науковий вісник Національного університету біоресурсів і природокористування України. Серія : Технологія виробництва і переробки продукції тваринництва - Year 2017, Vol 1, Issue 271
Abstract
The results of investigations of polymorphism of the BMP15 locus associated with the prolificacy of sheep are presented. The Polymerase Chain Reaction of the Polymorphism of Lengths of the Restriction Fragments (PCR-PLRF) was used for the studing on the sheep of the Ascanian Fine Fleece, Ascanian Karakul, Ascanian Meat-and-Wool and Romanov Breeds. The allotment of genomic DNA was performed using a set of Sorb-B (Amplicens) DNA reagents according to manufacturer's recommendations. PCR was performed using the programmable amplifier Lab Line (Germany). The restriction Mph11031 (recognition site - ATGCA↓T) was used for restriction. To separate the products of amplification and restriction, horizontal electrophoresis was performed in 2 % agarose gel with the addition of bromide ethidium. The visualization of the obtained results was carried out using transilluminator in ultraviolet light, by followed documentation of the electrophoregrams by a digital camera. The length of the amplification product of the BMP15 gene is 356 pairs of nucleotides. In the presence of a mutation in this locus, the enzyme Mph11031 cannot recognize the restriction site. However, in the absence of a mutation, the enzyme recognizes the restriction site and divides the amplified fragment into two segments of length 152 and 204 pairs of nucleotides. In the sheep of all the breeds studied, the BMP15 gene showed no mutation, which leads to an increase in prolificacy. All animals have a homozygous genotype ++, which corresponds to the wild type of sheep. The relevance of the conducted studies is that at present in Ukraine, the presence of a mutation in the BMP 15 locus on the sheep of the Southern region of Ukraine has not been studied yet. The aim of the research is to reveal the polymorphism (if any) of the gene BMP15 in the sheep of the Ascanian Fine Fleece, Ascanian Karakul, Ascanian Meat-and-Wool and Romanov breeds using the PCR-PLRF method. In future studies, it is planned to study the polymorphism of the GDF9 gene.
Authors and Affiliations
N. B. Pysarenko
Keywords
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