The Toxic Effects of Lead on Testicular Macrophage Immunomodualation and Sperm Cell Parameters in Mice
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 9, Issue 5
Abstract
Aims: In the present study the toxic effects of lead was investigated experimentally on the testicular macrophages and sperm cells isolated from testes of adult male mice to ascertain the extent of immunomodulation and reproductive dysfunctions (in-vivo). Study Design: Experimental study. Place and Duration of Study: Department of Biotechnology, Assam University, Silchar, Assam, India, between March 2013 and August 2014. Methodology: Dose response study was carried out with an increasing concentration of lead acetate. Percent mortality was determined for these doses and plotted graphically against the respective doses. From the graphs, LD50 value was determined. To validate immunomodulation of testicular macrophages and reproductive dysfunction due to lead intoxication, mice were divided into two groups. One group is treated with lead acetate (10 mg/kg body weight) and the other group with isotonic saline solution for 15 days. The isolated testicular macrophages were used to study the phagocytic property, alteration of enzyme release, cytokine release assay and the sperm cell were used for studying the sperm parameters in both control and treated group. Results: From the study significant decrease in phagocytic index (25516.61±1352.69 to 5154.67±437.37), myeloperoxidase release (77.3±10.7μM to 23.6±4.9μM), nitric oxide release (9.2±1.13 to 4.7±1.69) and a concomitant rise in the pro-inflammatory cytokine TNF-α were observed. These leads to an increased oxidative stress and inflammatory damage in the testes and subsequently less sperm count (78±1.155 to 24.33±1.764), sperm motility and abnormal sperm morphology was documented. Conclusion: Thus it could be concluded that the toxic potential of lead diminished the functional capacities of testicular macrophages, led to immunomodulation and inflammatory damage in testes and thus impede the sperm function parameters, which bear particular significance in heavy metal induced immune infertility in male.
Authors and Affiliations
Shamim Ahmed S. K. Barbhuiya, Mahuya Sengupta
Keywords
Related Articles
- EP ID EP347698
- DOI 10.9734/BJMMR/2015/17937
- Views 73
- Downloads 0
Is Salbutamol and Adrenalin Inhalation Effective in Management of Transient Tachypnia of Newborn?
Background: In the neonatal period, transient tachypnea of the newborn (TTN) is the most frequent cause of early respiratory distress because of delayed resorption of lung fluid, which fills the fetal airways. The inabil...
Prevalence of Disseminated Cryptococcosis among Human Immunodeficiency Virus Infected Patients in Benin City, Nigeria
Aim: To determine the prevalence of disseminated cryptococcosis among symptomatic HIV-infected patients, attending the Antiretroviral Treatment Clinic at the University of Benin Teaching Hospital, Benin City, Nigeria. St...
Use of Homeostatic Model Assessment Indexes for the Identification of Metabolic Syndrome and Insulin Resistance among Cuban-Americans: A Cross Sectional Study
Aim: to determine cut off points for The Homeostatic Model Assessment Index 1 and 2 (HOMA-1 and HOMA-2) for identifying insulin resistance and metabolic syndrome among a Cuban-American population. Study Design: Cross sec...
Association of Helicobacter pylori Infection with Colon Polyp and Colorectal Cancer
Background/Objective: Helicobacter pylori (Hp) is an etiology of gastrointestinal problems like gastric cancer. Its role in colorectal cancer is under investigations. Therefore, in this study we proposed the association...
Unsedated Colonoscopy: Experience from an Endoscopy Centre
Aim and Objective: Colonoscopy is generally considered a painful procedure requiring sedation. Due to the high cost of sedation colonoscopy, coupled with the attendant morbidity and mortality, there is a general trend to...