The Using of BAT25 Marker for Detection of Microsatellite Instability in Malignant and Benign Breast Tumors
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
:Microsatellites are small DNA sequences repeat (1 to 6 base pairs in length) spread throughout the genome. Microsatellite instability (MSI) is the spontaneous loss or gain of nucleotides from repetitive deoxyribonucleic acid (DNA) segments. This study was aimed to detect MSI in benign breast tumor (fibroadenoma) and breast cancer (ductal carcinoma) by using BAT25 marker. The formalin fixed paraffin embedded (FFPE) samples were taken from 21 cases with benign tumor and 23 cases with malignant breast tumor. Patients’ age range was 16-72 year with mean of (43 ± 2.1 SEM. years). Tumor and corresponding normal DNA of the same patient was extracted from about 25mg of FFPE samples. Conventional PCR was used for amplification of BAT25 locus the detection of MSI was done by using high resolution (HR) agarose. The results revealed that the incidence of at BAT25 locus in benign cases was (14%), while the incidence of MSI at BAT25 locus inmalignant cases was (13%). Moreover, no significant (P> o.05) correlation was found between MSI and age. As a conclusion, MSI represented by BAT25 marker can be detected in benign and malignant breast tumor.
Authors and Affiliations
Basman F. Abbas, Nazar A. Alwakeel, Nidhal A. Mohammad
Keywords
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- EP ID EP392086
- DOI 10.9790/3008-1205077380
- Views 103
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