The Very Low Frequency of Epstein-Barr JC and BK Viruses DNA in Colorectal Cancer Tissues in Shiraz, Southwest Iran
Journal Title: Polish Journal of Microbiology - Year 2018, Vol 67, Issue 1
Abstract
Viruses including Epstein-Barr virus (EBV), JCV and BKV have been reported to be associated with some cancers. The association of these viruses with colorectal cancers remains controversial. Our objective was to investigate their infections association with adenocarcinoma and adenomatous polyps of the colon. Totally, 210 paraffin-embedded tissue specimens encompassing 70 colorectal adenocarcinoma, 70 colorectal adenomatous and 70 colorectal normal tissues were included. The total DNA was extracted, then qualified samples introduced to polymerase chain reaction (PCR). The EBV, JCV and BKV genome sequences were detected using specific primers by 3 different in-house PCR assays. Out of 210 subjects, 98 cases were female and the rest were male. The mean age of the participants was 52 ± 1.64 years. EBV and JCV DNA was detected just in one (1.42%) out of seventy adenocarcinoma colorectal tissues. All adenomatous polyp and normal colorectal tissues were negative for EBV and JCV DNA sequences. Moreover, all the patients and healthy subjects were negative for BKV DNA sequences. The results suggested that EBV and JCV genomes were not detectable in the colorectal tissue of patients with colorectal cancer in our population. Hence, BKV might not be necessitated for the development of colorectal cancer. The findings merit more investigations.
Authors and Affiliations
Jamal Sarvari, Shahab Mahmoudvand, Neda Pirbonyeh, Akbar Safaei, Seyed Younes Hosseini
Keywords
Related Articles
- EP ID EP270369
- DOI 10.5604/01.3001.0011.6146
- Views 77
- Downloads 0
Relapsing Bacteraemia due to [i]Corynebacterium striatum[/i] in a Patient with Peripheral Arterial Disease
We describe the first reported case of [i]Corynebacterium striatum [/i]([i]C. striatum[/i]) relapsing bacteraemia in a patient with peripheral arterial disease and proven [i]Corynebacterium[/i] species colonization...
<strong>Monitoring of Virulence Genes, Drug-Resistance in <em>Campylobacter coli</em> Isolated from Golden Retrievers</strong>
The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (control). Probiotic preparation was administered on 43 day of the puppies life and 10 da...
MALDI-TOF MS Detection of Endophytic Bacteria Associated with Great Nettle (Urtica dioica L.), Grown in Algeria
Any plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. Th...
Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains
β-D-glucosidase (βG) gene from [i]Oenococcus oeni [/i]SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. Results showed that βG gene of the two strains was...
Endophytic Detection in Selected European Herbal Plants
A total of 181 cultivable endophytic bacterial isolates were collected from stems of 13 species of herbs inhabiting Europe (Poland): Chelidonium majus L., Elymus repens L., Erigeron annuus L., Euphrasia rostkoviana Hayne...