To Study the Effects of Various Fixatives on Liver – A Histological Study
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 12
Abstract
Abstract: Thefixation also in optical differentiation of cells and tissue constituents by altering their refractive indices in varying degrees. This is of value in the microscopic examination of cells and tissue.The present study aimed to find the best fixative for a particular organ, so that the best histological section details can be produced. We studied the effect of five different types of fixatives. An essential part of all histological and cytological techniques is preservation of cells and tissues as they naturally occur. To accomplish this, tissue blocks, sections or smears are usually immersed in a fixative fluid, although in the case of smears, merely drying the preparation acts as a form of preservation. The aim of the current study is to see the effect of the following fixatives namely 10% formalin, Buffered 10% formalin, Bouin’s fluid, Zenker’s fluid, Carnoy’s fluid on liver tissues and to observe the optimum result in a particular fixative in H&E sections. There is no single fixative which can be considered as best fixative for all purposes. Best fixatives for architectural preservation are Carnoy’s fluid and Zenker’s fluid. Best fixative for study of nuclear details is Bouin’s fluid. Keywords: Various fixatives, Liver, histological study.
Authors and Affiliations
Ved Prakash, Muktyaz Hussein
Keywords
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