To study the Photodynamic antimicrobial activity of Henna extract and preparation of topical Gel formulation
Journal Title: The Journal of Phytopharmacology - Year 2018, Vol 7, Issue 3
Abstract
Henna, known to possess antibacterial & antifungal activities is reported to contain naphthoquinone derivatives which have potential as photodynamic agents. Current study comprises of evaluation of Photodynamic antimicrobial activity of alcoholic extract of henna, followed by preparation & evaluation of a topical gel formulation. The alcoholic extract prepared by maceration was analyzed for phytochemicals. Lawsone (2- Hydroxy-1,4 naphthoquinone) was isolated from Henna and was characterized by UV, IR & MASS to confirm its structure. The HPTLC fingerprinting of extract and quantification of lawsone in extract and fraction was carried out. The effect of light was tested on extract and fraction to assess its photosensitizing property. The antimicrobial activity of the extract was tested where one set was exposed to light and other kept in dark and zone of inhibition calculated for both sets. The yield of fraction was found to be 0.5±0.1% w/w. The quantity of lawsone in alcoholic extract & fraction was found to be 1.3 ± 0.02 % w/w and 59.57 ± 1.01 % w/w respectively. The extract is nearly 75% as active as standard Gentamicin and the activity gets 20% potentiated on exposure to light for all test samples indicating Photodynamic antimicrobial activity. The gel was evaluated for its stability with developed HPTLC method and also for physicochemical parameters. The gel containing henna extract, exhibiting good Photodynamic antifungal & antibacterial properties, was found to be stable. The current study thus has resulted in developing a potentially effective herbal topical formulation for management of skin, scalp & hair infections.
Authors and Affiliations
Savita Kulkarni, Vanita Kale, Ketki Velankar
Keywords
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