Tocotrienol-Rich Fraction from Palm Oil Prevents Oxidative Damage in Diabetic Rats
Journal Title: Sultan Qaboos University Medical Journal - Year 2014, Vol 14, Issue 1
Abstract
Objectives: Tis study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. Methods: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. Results: FBG levels for the STZ-induced diabetic rats were signifcantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also signifcantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabetic rats when compared to the control group and diabetic group with TRF. Te results of the superoxide dismutase and glutathione peroxidase activities were signifcantly lower in the STZ-induced diabetic rats than in the normal group (P <0.001). Te levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were signifcantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabetic rats treated with TRF. Conclusion: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabetic rats.
Authors and Affiliations
Fatmah A. Matough| Department of Biology, Faculty of Science, Sabha University, Sabha, Libya, Siti B. Budin| Programme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia, Zariyantey A. Hamid| Programme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia, Mariati Abdul-Rahman| Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia, Nasar Al-Wahaibi| Department of Pathology, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, Oman, Jamaludine Mohammed| Programme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia
Keywords
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