Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest
Journal Title: Journal of Plant Protection Research - Year 2017, Vol 57, Issue 1
Abstract
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV-infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by the antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant protection against insect pests.
Authors and Affiliations
Volodymyr V. Oberemok, Kateryna V. Laikova, Alexey S. Zaitsev, Palmah M. Nyadar, Yuri I. Gninenko, Vladimir A. Gushchin, Valentin V. Makarov, Alexey A. Agranovsky
Keywords
Related Articles
- EP ID EP281884
- DOI 10.1515/jppr-2017-0003
- Views 62
- Downloads 0
Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis
Morphological and molecular diversity among 11 populations of cereal cyst nematodes from different wheat production areas in Morocco was investigated using light microscopy, species-specific primers, complemented by the...
Overwintering form of Erysiphe necator, the causal agent of grapevine powdery mildew in southern Syria
The main aims of this study were to determine the overwintering form and the primary inoculum of Erysiphe necator, the causal agent of grapevine powdery mildew in southern Syria. Eleven vineyards located at five differen...
Components of quantitative resistance in barley plants to Fusarium head blight infection determined using three in vitro assays
Quantitative resistance in barley to four Fusarium head blight (FHB) species was investigated in vitro. Nine components involved in three assays (detached leaf, modified Petri dish and seedling tests) were compared on tw...
Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate
Beauveria bassiana is an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determi...
Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy
The present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) using Trichodesma indicum aqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-...