UJI AKTIVITAS ANTIBAKTERI INFUSA DAUN SIRSAK (Annona muricata L.) SECARA in Vitro TERHADAP Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218 SERTA PROFIL KROMATOGRAFI LAPIS TIPISNYA
Journal Title: Kes Mas: Jurnal Fakultas Kesehatan Masyarakat - Year 2010, Vol 4, Issue 3
Abstract
Background: A research has been done to test the In vitro antibacterial activities of sirsak leaf (Annona muricata L.) infuse toward Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 to analyze its thin layer chromatography profile. This research done to observe the antibacterial activity from sirsak leaf infuse. Method: A test on the antibacterial activity was done by using liquid dilution method. The concentration infuse in sterile destilate water using to test the antibacterial activity toward S. aureus were 100%, 95%, 90%, 85%, 80%, and 75% w/v, while toward E. coli were 100%, 90%, 80%, 70%, 60%, and 50% w/v. To detect Chemical contains of the sirsak leaf infuse were identified using Screening method of Phytochemistry and Thin Layer Chromatography (TLC). Result: The result showed that the Minimum Bacterial Concentration (MBC) of sirsak leaf infuse S. aureus were 85% w/v, while E. coli could not be show antibacterial activity until 100% w/v concentration. The Minimum Inhibition Concentration (MIC) could not be identified by the liquid dilution because the mixture were turbid and the colour is dark brown. Screening method of Phytochemistry use tube-test and Thin Layer Chromatography showed that the infuse contain flavonoid, poliphenol, and alkaloid. Conclusion: Infuse of sirsak leaf (Annona muricata L.) has antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 profil infuse. Thin Layer Chromatography showed that the infuse contain flavonoid, poliphenol, and alkaloid.
Authors and Affiliations
Yeni Dianita Sari, Sitti Nur Djannah, Laela Hayu Nurani
Keywords
Related Articles
- EP ID EP275961
- DOI 10.12928/kesmas.v4i3.1093
- Views 79
- Downloads 0
Hubungan Perilaku Pencarian Pengobatan dengan Tuberkulosis Paru di Sulawesi Utara
Background: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Health seeking behavior is one of the factors that influence the incidence of pulmonary tuberculosis. The purpose of this study...
EFEKTIVITAS PENGGUNAAN LEACHET HASIL PENGURAIAN SAMPAH DALAM PROSES BIODEGRADASI LIMBAH BATIK
Background: Treatment of textile waste industry, including batik can be implemented with a biodegradation. Biodegradation by bacteria carried either aerobic or anaerob. The bacteria are widely found in nature. On the oth...
THE IMPORTANT ROLE OF LACTATING CORNER AT PKU MUHAMMADIYAH YOGYAKARTA, INDONESIA
Background: Breastfeeding offers special health benefits to both infant and mother. A breastfeeding-friendly hospital might be an essential factor for predicting breastfeeding rates among women. Method: explored the imp...
HUBUNGAN ANTARA ASUPAN MAKANAN DAN STATUS KESADARAN GIZI KELUARGA DENGAN STATUS GIZI BALITA DI WILAYAH KERJA PUSKESMAS SEWON I, BANTUL
Background: Poor nutritional status of children in Puskesmas Sewon I in 2007 amounted to 0.60%. Family Nutrition Awareness Status in Puskesmas Sewon I was at 47.06%. Average consumption of energy and protein adequacy of...
ANALYSIS OF SECTIO CAESAREA DELIVERY AT RSUD SRAGEN, INDONESIA
Background: Based on data in Sragen District, 2010 showed that AKI (mother mortality rate) written 69.7/10.000 live births and AKB (baby mortality rate) noticed 6.8/100.000 live births. Caesarean Section method was on of...