Upregulation of GADD45α, TP53 and CASP3 mRNA Expression in the Rat Lens after In vivo Exposure to Sub-threshold Dose of UVR B
Journal Title: Journal of Ocular Biology - Year 2014, Vol 2, Issue 1
Abstract
Purpose: The objective of the present study was to investigate the evolution of mRNA expression of the stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens after in vivo exposure to sub-threshold dose of UVR-B. Methods: Forty six-week-old female albino Sprague-Dawley rats were unilaterally exposed to a sub-threshold dose, 1 kJ/m2 (1.1 W/m2 for 15 min), of UVR (λmax = 300 nm). Anesthetized and dilated eyes were exposed to UVB radiation. The animals were sacrificed at 1, 5, 24 and 120 h post-exposure. mRNA expression of the GADD45α, TP53 and CASP3 genes in the lenses was measured by quantitative RT-PCR, and fold change in mRNA expression between exposed and unexposed lenses was calculated. Results: mRNA expression for GADD45α increased to a 1.2 fold change at 1 h after exposure and then returned to no change at 120 h. mRNA expression for TP53 increased with a regression coefficient of 0.04 h-1 to a maximum of 1.67 fold change. mRNA expression for CASP3 increased with a regression coefficient of 4.5 x 10-3 rel. units/h to a 1.46 fold change at 120 h after exposure. Conclusions: A sub-threshold in vivo exposure to UVR-B causes a transient upregulation of the stress sensor GADD45α at 1 h after exposure, a saturating upregulation of TP53 and a subsequent constant upregulation of CASP3 in the rat lens.
Authors and Affiliations
Konstantin Galichanin
Keywords
Related Articles
- EP ID EP197780
- DOI 10.13188/2334-2838.1000016
- Views 130
- Downloads 0
Unilateral Acute Idiopathic Maculopathy Associated with Hand-Foot-Mouth Disease
Unilateral acute idiopathic maculopathy (UAIM) is a rare disorder that has been linked with coxsackie virus. A viral prodrome is common. We aim to add to the literature a case of challenging visual symptoms associated w...
Delayed Branch Retinal Artery Occlusion and Partial Oculomotor Nerve Palsy Following Coiling of a Giant Intracavernous Carotid Artery Aneurysm
Coil embolization is a non-invasive and effective method of treating intracranial aneurysm s not amendable to surgical clipping by inducing thrombus formation. However, ophthalmic complications and cranial nerve injury a...
Evaluation of Dry Eye in patients with Psoriasis
Purpose: To evaluate the role of routine tear film tests such as Schirmer, tear film break up time (TBUT), Rose Bengal staining (RBS) and conjunctival impression cytology for dry eye evaluation in patients with psoriasis...
The Existence of mRNAs and miRNAs Expressions for Maintaining Cell Survival Networks Associated with the Human Transparent and Cataractous Lens
Background: Human lens cells have a long lifespan and so must maintain cell survival networks with regulate biological processes including gene expression and regulation. The purpose of this study was to identify mRNA tr...
Overview of Congenital, Senile and Metabolic Cataract
Opacity of the lens is a major public health problem of unknown causes and impaired vision of a large number of peoples. There are different forms of cataractous lenses including congenital, senile and metabolic associat...