Use of AFLP markers for molecular characterization of two crops with agricultural interest
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 2
Abstract
The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA), adapter ligation with fragments of DNA and amplification of the fragments obtained in the earlier steps using combinations of selective primers and detection of polymorphism using radioisotopes, fluorescent dyes or silver staining. It is widely used in the evaluation of genetic diversity, analysis of genetic distance, DNA fingerprint, germoplasm collections analysis, genetic maps construction and markers diagnostic monitoring. In order to characterize molecularly two species of crops with agricultural interests (sugar cane and banana) were generated AFLP markers. In this study was demonstrated the ability to identify variations in the genome of crops studied
Authors and Affiliations
Luis E. Rojas, Jorge López , Rafael G. Kosky , Orelvis Portal
Keywords
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