Use of cytochrome oxidase 1 gene region: a molecular tool for the domestic and wildlife industry in Kenya
Journal Title: Scientific Journal of Animal Science - Year 2014, Vol 3, Issue 3
Abstract
Illegal substitution of meat products by traders either as closely related domestic species or as bush meat is a common occurrence in various parts of Kenya. This has implications on biosafety, food safety and consumer confidence and subsequently the meat and meat products industry both locally and export. In recent times, use of molecular techniques have seen increased application in wildlife conservation through conservation genetics in areas such as population genetics, evolutionary genetics, molecular ecology and wildlife forensics. We used DNA of the Cytochrome C Oxidase 1 gene region as a bar-coding technique for species identification. The accuracy of CO1 as a marker was tested using five known samples of wildlife species.Retail meat product substitution and bushmeat prevalence was estimated from 99 unknown meat samples that were randomly collected from meat traders in Nakuru County. The study validated the use of CO1 marker for species identification and illustrated use of the marker in identification of unknown tissue samples collected from the market survey.
Authors and Affiliations
D. Mbugua*, C. N. Kimwele, J. O. Jung’a, E. Gatebe, F. Gakuya, M. J. Kinuthia, M. M. Mugambi, E. S. Kwamboka
Keywords
Related Articles
- EP ID EP683
- DOI 10.14196/sjas.v3i3.1137
- Views 492
- Downloads 23
Effect of Imbrasia belina (westwood), Tylosema esculentum (Burchell) Schreiber and Vigna subterranea (L) Verde as protei
A feeding trial was conducted to determine growth and laying performance of indigenous Tswana hens fed diets formulated with four different protein sources consisting of either Tylosema esculentum (Burchell) or Vigna s...
Effects of Goldfish (Carassius auratus) and Roach (Rutilus rutilus) extracts on the growth indexes and survival rate of
In order to investigate the effect of extracts of Goldfish and Roachextracts on the food acceptability of giant sturgeon fingerlings (Huso huso),an experiment for about a month was carried out in the Aquaculture Resear...
Supplementing panicum maximum with two medicinal forages in the diet of Djallonke sheep at the Benin national sheep cent
Ensuring a better integration of natural resources in the animal feeds remains the main challenge that farmers face most of the time. The present study, conducted in the breeding farm of Betecourou, deals with the nutr...
Seed dispersal by serrated tortoises (Psammobates Oculiferus) and the effect of their gut passage on seed germination
Some tortoises are frugivores and can disperse seeds away from the parent plant to areas favourable for their establishments through gut passage. We investigated the plant species dispersed by the serrate...
Development and promotion of village chickens subgroups to counteract the adverse effects of climate change in Sub Saharan Africa
Smallholder agriculture sector which harbors different village chickens ecotypes/varieties/strains is one of the most susceptible to the adverse effects of climate change due to its low adaptive potentiality. As of now,...