Utility of C-Kit Expression by Immunohistochemistry as a Prognostic and Theranostic Marker in Colorectal Carcinoma
Journal Title: Indian Journal of Pathology and Oncology - Year 2017, Vol 4, Issue 3
Abstract
Objectives: Targeted therapy is now available for various carcinomas with growth factor receptor mutations. C-Kit, a tyrosine kinase receptor is overexpressed in many epithelial malignancies. The aim of this study is to determine the role of c-Kit (CD117) expression by Immunohistochemistry (IHC) in colorectal carcinoma (CRC) and to correlate the same with tumor grade, stage, nodal status and metastasis. Methods: This is a retrospective study conducted on paraffin blocks of histopathology proven CRC. Monoclonal antibody detected against c-kit antigen was observed in the cytoplasm and epithelial membrane of the tumour cells and c-Kit expression was given a score based on staining intensity and percentage of cells showing positivity. A statistical analysis using chi square test was performed to find any difference between the various tumor grade, stage, nodal status and metastasis with respect to their c-kit expression by IHC. Results: c-Kit was negative in 55% of the cases and out of 45% positive cases 3+ positivity was noted in 5% of the cases only. 2+ positivity was seen in 9% and 1+ in 31%. Statistical data showed a p-value of 0.96, 0.70, 0.48 and 0.68 for grade, stage, nodal status and metastasis of the tumor with respect to c-kit expression respectively. Conclusion: C-kit expression by IHC has only a limited role and is statistically not significant as a prognostic or theranostic marker in CRC. Molecular analysis of the tyrosine kinase receptor mutation is needed to determine its aberrant expression.
Authors and Affiliations
G. Barathi, Leena D. Joseph, S. Rajendiran, Suresh Varadarajan
Keywords
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- EP ID EP315368
- DOI 10.18231/2394-6792.2017.0082
- Views 76
- Downloads 0
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