Utility of manually constructed large core micro-array for routine immunohistochemical assessment of ER, PR and HER2 in carcinoma breast
Journal Title: Indian Journal of Pathology and Oncology - Year 2017, Vol 4, Issue 1
Abstract
Aim: To compare Immunohistochemistry (IHC) results of large core Tissue micro array (TMA) with the conventional whole section for estrogen receptor (ER), progesterone receptor (PR) and HER2 expression in breast carcinoma cases. Methods: We analyzed 51 breast carcinoma cases. Large core TMAs were manually constructed with easily available materials. Immunohistochemical evaluation of ER, PR and HER2 were carried out in parallel by TMA and whole section methods for all cases as per Allred scoring system. Observed IHC scores were compared. Results: We detected a high concordance rate of 98%, 100% and 98% for ER, PR and HER2 respectively between TMA and whole sections. In addition, the use of TMA caused a significant reduction in the use of laboratory resources- reagents, slides and blocks. Conclusion: Our analysis showed various advantages of TMA over whole sections in IHC assessment. Hence, TMA can be implemented as a valuable tool in the routine assessment of breast biomarkers without compromising quality.
Authors and Affiliations
Sunilkumar KB, Nikethan B, Harini R
Keywords
Related Articles
- EP ID EP311458
- DOI 10.18231/2394-6792.2017.0020
- Views 64
- Downloads 0
Spectrum of breast lesions and cyto- histopathological correlation - A retrospective study in a teaching institution in North Malabar
Introduction Breast carcinoma is the most common malignancy in women Fine needle aspiration Cytology FNAC is an important component of triple approach for diagnosing breast lesions and is highly sensitive specific rapid...
Study of awareness about early detection of cervical cancer by PAP smear screening amongst women of Bhavnagar District
Introduction: Cervical cancer is one of the most common cancers in women worldwide. In India, cervical cancer is the most common woman-related cancer, followed by breast cancer. 80% of the new cervical cancer cases occur...
Histopathological evaluation of cerebral lesions
Introduction A cerebral lesion refers to any type of abnormal tissue found in the brain particularly in the cerebral cortex The cerebrum is responsible for allowing voluntary movements within the human body Cerebral tumo...
Histopathologic correlates of clinically verrucous lesions
Introduction Verrucous lesions present as cauliflower like slowly growing masses which may be single multiple or diffuse involving the broad areas of skin or mucosal surfaces Clinically these lesions are diagnostic chall...
Diagnosis of Cysticercosis in post auricular swelling on FNAC- A case report
Introduction Cysticercosis a parasitic tissue infection is caused by larva of Taenia solium Cysticercus cellulosae Fine needle aspiration cytology FNAC provides direct specific simplest and early diagnosis of cysticercos...