UV-Visible spectrophotometric detection of Solanum aethiopicumhexanic and n-butanolic excerpts and their anticancer activity monitored by flow cytometry and microscopy
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2016, Vol 5, Issue 3
Abstract
UV-Visible Spectrophotometry (UV-Vis) is one of the most used analytical technics because of its accuracy, however, it application in phytochemicals and extracts characterization still a huge challenge even for the choice of appropriate wavelength at HPLC detector. TLC screening of Solanum aethiopicumL.(Solanaceae) hexanic and n-butanolic excerpts respectively F1, F4 has been reported but these extracts have never been characterized by UV-Vis. Likewise the study of their potential effect against cancer cells using microscopy and flow cytometry has never been performed. The aim of this work was firstly to characterize F1 and F4 by UV-Vis, find out the possible embedded phytochemicals and secondly to assess their anticancer potential. The findings show that F1 has 33 major phytocompounds detected from 176-254 nm while F4 contained 45 detected between λ 176-679 nm and mostly identified as sterols, terpenes, phenolic acids and flavonoids which may be responsible oftheiranti-proliferative effects againstJurkat cells (Leukemia cancer).
Authors and Affiliations
Kouassi Kouadio Christian*| UFR-SFA,Bioorganic Chemistry and Natural Substances Laboratory, NanguiAbrogoua University 02 BP 801 Abidjan 02, Côte d’Ivoire., Sorokina Elena Anatolievna| Faculty of Physical, Mathematical and Natural Sciences, Department of Organic Chemistry, Organic Synthesis Laboratory, Peoples’ Friendship University of Russia, 6 Milklukho-Maklaya St., Moscow, Russia 117198., Mamyrbékova-BékroJanat Akhanovna| UFR-SFA,Bioorganic Chemistry and Natural Substances Laboratory, NanguiAbrogoua University 02 BP 801 Abidjan 02, Côte d’Ivoire., Békro Yves-Alain| UFR-SFA,Bioorganic Chemistry and Natural Substances Laboratory, NanguiAbrogoua University 02 BP 801 Abidjan 02, Côte d’Ivoire.
Keywords
Related Articles
- EP ID EP3731
- DOI -
- Views 389
- Downloads 18
Optimization of dispersive liquid-liquid microextraction followed by flame atomic absorption determination of Cu (II), Ni (II) and Co (II) based on the complexation reaction with phenathrene-9, 10-dine dioxime (PD)
In this study, a dispersive liquid-liquid microextraction (DLLME) method was used for preconcentration of Cu (II), Ni (II) and Co (II) cations from water samples. Oxime (phenathrene-9, 10-dine dioxime (PD)) was used as...
In Vitro Cytotoxicity Assessment of Imiquimod Loaded Liposomal Gel Formulation Using Basal Cell Carcinoma Cell Line: Part-II
This study was undertaken to address the efficacy of newly formed Imiquimod loaded liposomal gel formulation. The study evaluated the acute toxic effect of liposomal gel formulation intended to be used in skin cancers li...
Colonization of Beneficial Microflora in the GUT of Indian Adult Females (35-50 yrs) as Affected by Dietary Fibre Intakes. Short Running Title: Gut Health and Dietary Fiber
BACKGROUND AND OBJECTIVES: Probiotics and prebiotics have recently been recognized as important functional foods that enhance the establishment of beneficial bacteria in the human gut which helps in the prevention of lif...
SEROPOSTIVITY OF TRANSFUSTION TRANSMISSIBLE HIV INFECTIONS AMONG VOLUNTARY AND REPLACEMENT BLOOD DONORS
Background: The evaluation of the data of the prevalence of transfusion transmissible infections (TTI) among blood donors permits an assessment of the acqisition of the infections in the blood donor population and conseq...
PREPARATION AND EVALUATION OF IMIQUIMOD LOADED SOLID LIPID NANOPARTICLES BASED TOPICAL GEL FORMULATION: PART-I
Solid lipid nanoparticles are at the forefront of the rapidly developing field of nanotechnology with several potential applications in drug delivery, clinical medicine and research as well as in other varied sciences....