Validated RP-HPLC method for estimation of Etoricoxib & Paracetamol and its applications in analysis of formulations
Journal Title: Journal of Pharmacology and Biomedicine - Year 2017, Vol 1, Issue 1
Abstract
The current paper describes a reversed phase high performance liquid chromatographic method for the simultaneous estimation of Etoricoxib and Paracetamol in formulations. The separation was achieved on the LUNA C18 column 5µ (250 x 4.6 mm id), using methanol - water in the ratio 63 - 37 as the mobile phase at 1 ml/min flow rate and 237 nm as detection wavelength. The retention time of Etoricoxib and Paracetamol were 1.6 and 2.9 min respectively. The method was validated in terms of linearity, accuracy, precision, as per ICH Guidelines. The calibration curve was linear in the concentration range from 10-60 µg/ml for etoricoxib and 100-600 µg/ml for paracetamol. Percentage recovery obtained for etoricoxib and paracetamol were 99.60 % and 99.04 % respectively.
Authors and Affiliations
Prakash Sahu, Shweta Singh
Keywords
Related Articles
- EP ID EP251201
- DOI -
- Views 128
- Downloads 0
Derivatization of Gallic Acid with amino acids for accentuation of its antioxidant potential
Phenolics like gallic acid have been known for their anti oxidant activity since long time. Many attempts for increasing antioxidant potency of phenolics have been made utilizing synthetic and drug discovery tools. In an...
Total phenolic content, flavonoid concentration, antimicrobial and insecticidal screening of aqueous extracts of Annona squamosa (seeds), Azadirachta indica (leaves) and Lavandula angustifolia (flower)
The present study describes the total phenolic content, flavonoid concentration, antimicrobial and insecticidal screening of aqueous extracts of Annona squamosa (seeds), Azadirachta indica (leaves) and Lavandula angustif...
An Evaluation of Phytochemicals in Maceration and Infusion Extracts of Cyperus rotundus Linn Leaves in Two Different Solvents
The objective of the study to evaluate the hepatoprotective effect of ethanolic extract of Curcuma caesia rhizomes in Wistar rats on paracetamol induced liver. Albino wistar rats were separated into five groups (n=6). Et...
Synthesis of 1,8-Napthyridine derivatives and their evaluation as possible antiepileptic agents
Aminopropyloxy derivatives of 1,8-naphthyridines have been synthesized using the principle of Conrad-Limpach synthesis of quinolines. The hydroxy group at 2- position of the naphthyridine ring was converted to the corres...
Drug Discovery Tools: Computer Aided Molecular Design and ChemoInformatics
The article reviews the different approaches used in computer aided molecular design. It also reviews current achievements in the field of chemo-informatics and their impact on modern drug discovery processes. The main d...