Validated RP-HPLC Method for Quantification of Paclitaxel in Human Plasma – Eliminates Negative Influence of Cremophor El
Journal Title: International Journal of Current Research and Review - Year 2018, Vol 10, Issue 13
Abstract
Background: Literature reports innumerable methods for quantification of paclitaxel in biological matrices. Most of these involve complicated extraction procedures like solid phase extraction, separate procedure for elimination of interference of Cremophor El, advanced and expensive instruments. Objectives: The objective of the present research work is to develop and validate a simple, rapid, sensitive, economic and reproducible reverse phase – high performance liquid chromatography method for the estimation of paclitaxel concentration in human plasma that eliminates negative influence of Cremophor El on recovery of paclitaxel. Methods: Chromatographic separation of paclitaxel was carried out using C18 column (150 × 4.6 mm i.d., 4μm particle size, Waters, Australia) with 60% acetonitrile, 40% of 10mM ammonium acetate buffer solution and 0.1% formic acid as a mobile phase at a flow rate of 1.0mL/min at ambient temperature. Validation was performed as per ICH Q2 guidelines. Results: In this system the retention time was 3min. The detection limit was 5ng/mL and limit of quantification with reproducibility was 15ng/mL. Plasma samples were extracted using single solvent (tertiary – Butyl Methyl Ether) liquid- liquid extraction with a recovery of 96-99%. Robustness of the method was established with variation in flow rate, detection wave length and mobile phase composition. Stability of paclitaxel during the study period was studied and found to be stable. Conclusion: The developed method is easy to perform, quick, reproducible with good recovery without negative influence of Cremophor El and applicable to quantify paclitaxel in regular clinical practice for individualization of the therapies.
Authors and Affiliations
Mangathayaru K.
Keywords
Related Articles
- EP ID EP533379
- DOI 10.31782/IJCRR.2018.10132
- Views 141
- Downloads 0
Correlation between the Type of Arterial Vascularizacion and Presence and Localization of Myocardial Bridges
Introduction: Myocardial bridges represent an anomaly of the coronary arteries’ flow, which can be asymptomatic, or can lead to the coronary insufficiency and its clinical consequences. The aim of this research was to de...
Magnetohydrodynamics (MHD) Forced Convective Flow and Heat Transfer Over a Porous Plate in a Darcy-Forchheimer Porous Medium in Presence of Radiation
Aim and Objective: An analysis is made to study the problem of boundary layer forced convective flow and heat transfer of an incompressible fluid past a plate embedded in a Darcy-Forchheimer porous medium in presence of...
Pharmacobiological Treatments in Autism Spectrum Disorders
Autism spectrum disorders (ASD) refer to a group of neuro-developmental disorders affecting young children and adults. Currently, the treatment options for ASD are mostly restricted to treating its symptoms. Among the va...
Visual Outcome and Complications of Resident Operated Phacoemulsification Cases Done by Third Year Residents in a Regional Institute of Ophthalmology
Introduction: Phacoemulsification is a state of the art surgery for cataract removal. Phacoemulsification involves removing the opacified crystalline lens via emulsification and aspiration followed by implantation of an...
A Study on Infrastructural Facilities for Differently Abled in Banks
Introduction: Differently abled is a term applied to all persons with disabilities including those having long term physical, intellectual impairment, mental impairments etc. Person with disability often struggle with th...