VALIDATED SPECTROPHOTOMETRIC METHOD TO DETERMINE VARDENAFIL AND SILDENAFIL IN PHARMACEUTICAL FORMS USING POTASSIUM IODIDE AND POTASSIUM IODATE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 10
Abstract
Objective: To develop and validate simple, sensitive, precise and free of organic solvents method for the determination of sildenafil (SIL) and vardenafil (VAR) in bulk and pharmaceutical formulation.Methods: The method is based on the reaction of studied drugs with a mixture of potassium iodide and potassium iodate in an aqueous medium at (25±2 °C) to form yellow coloured triiodide ions (I3-) within 45 min. The reaction is followed spectrophotometrically by measuring the absorbance at 288, 351 nm and 285, 351 nm for sildenafil and vardenafil respectively.Results: The effects of analytical parameters on the reported systems were investigated. Beer's law of SIL was obeyed in the range of (0.4-12) μg ml-1and (0.6-16) μg ml-1. Molar absorptivity was found to be (67.659 ×103) lmol/cmand (37.955×103) lmol/cm at 288 nm, 351 nm respectively. Beer's law of VAR was obeyed in the range of (0.2-13) μg/mland (0.5-40) μg/ml. Moreover, molar absorptivity’s were found to be (68.719 ×103) l mol-1 cm-1and (26.691×103) l mol-1 cm-1 at 285 nm, 351 nm respectively.Conclusion: The proposed method has been applied to determine the components in dosage forms with an average recovery of 98.15% to 103.45% and the results have been found in good agreement with those results obtained by the reference methods.
Authors and Affiliations
Amir Alhaj Sakur, Shaza Affas
Keywords
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- EP ID EP575449
- DOI 10.22159/ijpps.2017v9i11.20578
- Views 80
- Downloads 0
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