Validation of Sterility Assurance Level Up To 106 Log Reductions
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2017, Vol 3, Issue 12
Abstract
Abstract:An attempt was made to validate Sterility Assurance level (SAL) of two Industrial Autoclaves up to 106 Log Reduction by using both gram negative and gram positive bacteria. For gram negative bacteria, a suspension of E. coli was prepared at 108 cfu/ml which was sterilized individually through two autoclaves at 1210C for 20 minutes at three consecutive cycles. Bacterial load was then determined and found 8 log reductions of bacterial growth. For Gram positive bacteria, 12 ampoulesofGeobacillusstearothermophilus containing 106 cfu were placed in two Autoclaves. After sterilization, no growth of Geobacillusstearothermophilus was found which also proving the 106 Log Reductions of that sterilization parameters. Keywords:SAL, Log Reduction, Sterilization, Geobacillusstearothermophilus, Autoclave.
Authors and Affiliations
Reazul Karim, JobairHossain SM, Vahitha V, Kadar Basha S
Keywords
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