Abstract

Objective: To evaluate the contents of curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) and the cytotoxic activity in ethanolic extracts of 20 Curcuma aeruginosa RoxB. (C. aeruginosa) accessions collected from regions of Indonesia. Methods: Curcuminoid compounds were investigated by high performance liquid chromatography with standards, and the cytotoxic activity was evaluated by the brine shrimp lethality test. Results: Curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin contents of C. aeruginosa accessions varied from 0.01% to 1.95% with the coefficient of variation (CV) of 27.36%, 0.01% to 1.45% with a CV of 30.56%, 0.01% to 0.47% with a CV of 25.16%, and 0.01% to 0.03% with a CV of 30.90%, respectively, which was higher in the Wonogiri accessions. All accessions of C. aeruginosa were found to be effective in general toxicity against brine shrimps. Conclusions: The Wonogiri accession of C. aeruginosa could be selected as a high quality clone for curcuminoid production for an industry breeding program.

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