Virulence and antimicrobial resistance genes associated with the in-vivo pathogenicity of avian pathogenic E. coli isolates
Journal Title: GMPC Thesis and Opinions Platform - Year 2021, Vol 1, Issue 1
Abstract
In the current study, ten avian pathogenic E. coli (APEC) isolates of the most predominant APEC serogroups isolated from broiler chickens in Egypt were screened for their virulence and antimicrobial resistance genes pattern using PCR. Five selected virulence gene patterns were further investigated for their in-vivo pathogenicity test. Results showed a 100% prevalence of the β-lactams and tetracyclines resistance genes. However, aminoglycoside and quinolone resistance genes were not detected. Also, 80% of the tested isolates harbored mcr-1 gene, colistin resistance gene. In-vivo pathogenic strains consistently harbored the virulence gene pattern of fimH, fimA, papC, iutA, and tsh. Additionally, the tsh gene was consistently detected with lethal APEC isolates in day-old chicks. These results highlighted the high prevalence of antimicrobial and virulence genes in APEC that potentially represent a public health concern. In this study, the virulence genes fimH, fimA, papC, iutA, and tsh were the most common virulence gene patterns associated with pathogenicity in day-old chicks
Authors and Affiliations
Ahmed I. Abd El-Mawgoud, Azza A. A. El-Sawah, Soad A. Nasef, Al-Hussien M. Dahshan and Ahmed Ali
Keywords
Related Articles
- EP ID EP692689
- DOI https://doi.org/10.51585/gtop.2021.0005
- Views 130
- Downloads 0
Editorial: The GMPC Thesis and Opinions - A platform for science globalization
The research effort is effective when published and becomes available to the scientific community. Scientist's professional success correlates to a greater extent with their research "internationalization." Although many...
Avian influenza infections in poultry farms in Egypt, a continuous challenge: Current problems related to pathogenesis, epidemiology, and diagnosis
This study's main objective was to update the avian influenza (AI) epidemiological situation, including molecular characterization reassortment analysis and genotyping of circulating AI virus (AIV) subtypes in Egyptian p...
Virulence and antimicrobial resistance genes associated with the in-vivo pathogenicity of avian pathogenic E. coli isolates
In the current study, ten avian pathogenic E. coli (APEC) isolates of the most predominant APEC serogroups isolated from broiler chickens in Egypt were screened for their virulence and antimicrobial resistance genes patt...
Towards a novel bioreactor technology for treating fungicide-rich wastewaters from the fruit packaging industry
The fruit processing industry is an important economic sector worldwide, whereas a wide range of plant pathogens affects the shelf life of fruits, resulting in great economic losses. Post-harvest fungicides are often app...
COVID-19 situation in Baden-Württemberg, Germany: Preliminary case series study during the first wave
The present case series study presents the preliminary data of 347 of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) positively tested patients in the Mittelbaden hospital, Baden-Baden Bühl, Germany, d...