Vitamin D3 Supplement Rescues Motor Disability in 3-Nitropropionic Acid Induced Mouse Model of Huntington’s Disease
Journal Title: The 1st Annual Meeting of Georgian Center for Neuroscience Research - Year 2020, Vol 2, Issue 20
Abstract
Background: The major outcome of all neurodegenerative disorders including Huntington’s disease (HD) results in loss of brain cells called neurons. Neuronal loss in the striatum, the major brain region responsible for motor coordination results in Huntington’s disease (HD). HD results in motor disability and degeneration of striatal medium spiny neurons. In this regard, the preclinical evidence indicates that Vitamin D3 (VD) supplement delays neuronal loss and exerts neuroprotective effect in neurological disorders like Alzheimer’s disease and Parkinson’s disease. Rationale: In this study we want to explore if VD exerts its neuroprotective role in HD. We used 3-nitropropionic acid (3-NP) to induce striatal degeneration which is well established model of HD. Methods: We established a mouse model of HD by using 3-NP. Male C57BL/6 mice were divided into four groups (I-IV). Group I: Mice received saline intraperitoneally (vehicle). Group II: Mice induced with 3-NP (75mg/kg) Group III: Mice dosed with VD (500IU/kg) for 15 days. Group IV: 3-NP induced mice dosed with VD (500IU/kg) for 15 days. Various behavior tasks were studied to test motor functions like locomotion activity, stride analysis and rotarod analysis for all four groups for 15 days. Results: Our study demonstrate that 3-NP treatment significantly impaired locomotor activity and motor coordination when compared with vehicle treated animals. Animals treated with VD supplement (500IU/kg) showed significant improvement in the motor coordination and locomotion in 3-NP induced mouse model of HD when compared with control. Also, animals injected with only VD did not show any change in coordination or locomotion indicating that VD mediated signaling could rescue only when neurons degenerate. Conclusion: The present study suggest that VD supplementation could be used as an effective agent for neuroprotection and rescue motor disability in 3-NP mouse model of HD.
Authors and Affiliations
SKV Manjar, Sanjana Srinivas, PG Sathwik, Pragya Komal
Keywords
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- EP ID EP677910
- DOI 10.29088/GCNR-2020.01
- Views 289
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