ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 1
Abstract
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transforming growth factor β1(TGFβ1) and to explore its promoting femoral fracture healing in rats. Femur fractures were generated in 72 male adult Wistar rats by cutting femur transversely at middle point. ZJXG decoction was administered orally after surgery for 28d. The fracture healing process was analyzed by X-ray, gross anatomy and hematoxtlin-eosin (HE) staining. The serum levels of BMP-7, IGF-1, bFGF and TGFβ1 were detected by enzyme linked immunobsorbent assay (ELISA). X-ray imaging and gross anatomy indicated that the fibrous callus tissue at the femoral fracture-end increased and the fracture line became fuzzy at 7-14 d following treatment with ZJXG Decoction. HE staining showed that the fibrous-granular tissue at the fracture-end changed gradually to fibrous, cartilaginous and osseous callus tissues from 7d to 28d. ELISA results showed that the serum levels of BMP-7, IGF-1, bFGF and TGFβ1 increased significantly 28 days following treatment with ZJXG decoction, compared to model group. It is concluded that ZJXG decoction could enhance the fracture healing by reducing the decomposition of BMP-7 and IGF-1, bFGF and TGFβ1 and enhancing their activities.
Authors and Affiliations
Zhen Zhou, Chi Ning, Guanxi Wang, Yunliang Guo, Guangwen Yang
Keywords
Related Articles
- EP ID EP654403
- DOI 10.24297/jab.v5i1.5350
- Views 202
- Downloads 0
Qualitative and quantitative changes in the haemogram of desert locust Schistocerca gregaria (Orthoptera: Acrididae) by extracts of Nigella sativa (Ranunculaceae).
The present work was carried out aiming to assess the Nigella sativa extracts on some hematological parameters of nymphs and adults of the dangerous pest Schistocerca gregaria. Only three haemocyte types were recognized...
Population analysis of Tapes decussata (Bivalvia: Veneridae) in Lake Timsah, Suez Canal, Egypt
The population of Tapesdecussatawas abundant in the southern region of Lake Timsah. It was investigated between August 2004and September 2005. Mean abundance and biomass were 76.9 ind.m-2 and 5.33 g.m-2 (flesh dry weight...
Severity of Rice Disease Caused by Curvularia Spp In Malaysia
Curvularia is a hyphomycete fungus that is a facultative pathogen that affects many plants species especially in the tropical region. Rice researchers have documented Curvularia as one of the 6 main pathogenic fungi that...
Bifonazole in vitro activity and its azole-parallel resistance in clinical yeast isolates
The susceptibility/resistance profile of bifonazole (BFZ) in 170 dermatophyte strains including azole parallel-resistance in 324 clinical yeast isolates was determined, additionally with impact on patient-relevant factor...
Effects of radiation leakage of microwave oven on male Swiss albino mice
Continuous microwave radiation leakage from microwave oven may be harmful for users who have high exposure to them. The aim of this work was to assess the effects of microwave leakage on blood, liver, kidney and testis o...