牛磺酸拮抗锰诱导体外培养的皮层神经细胞凋亡
Journal Title: Science Paper Online - Year 2007, Vol 2, Issue 11
Abstract
【目的】研究牛磺酸对锰诱导体外培养的皮层神经细胞凋亡的拮抗作用。【方法】体外分离新生乳大鼠皮层神经细胞，培养至最佳生长状态时进行实验。细胞随机分为7组，即对照组、染锰组（设有低、中、高3个剂量）、干预组（3组，培养液同时加有锰及牛磺酸）。低、中、高锰的浓度分别为0.2mmol/L,0.6mmol/L和1.0mmol/L，牛磺酸的浓度为1.5mmol/L。干预24h后终止培养，用末端脱氧核苷酸转移酶介导的缺口末端标记(TUNEL)及流式细胞术(FCM)定性、定量检测神经细胞凋亡的变化。【结果】(1) TUNEL显示锰干预后神经细胞呈现典型的凋亡形态学变化：核固缩、碎裂，部分甚至核崩解，出现凋亡小体，且凋亡的神经数量有随着锰浓度升高而增多的趋势；正常细胞则染色质均匀，形态完整；牛磺酸干预对中低浓度锰有一定的拮抗作用，高浓度锰未见明显改善；(2) FCM定量研究表明锰诱导神经细胞凋亡与其浓度有剂量-依赖关系，牛磺酸能拮抗中低浓度锰所致的细胞凋亡，但对于过高浓度锰所致细胞急剧的死亡—坏死则无改善作用。【结论】锰可致体外培养的皮层神经细胞死亡（坏死或凋亡），牛磺酸对中低锰所致的凋亡有保护作用，但对于过高浓度锰致使神经细胞急剧坏死无保护作用。
Authors and Affiliations
Songchao GUO, Cailing LU , Weiping CHEN
Keywords
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