16S rDNA based identification of culturable bacteria from highly polluted Bangalore, India
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2017, Vol 25, Issue 1
Abstract
This study investigated the microbial population of highly polluted Varthur and Bellandur lakes, the largest freshwater lakes of Bangalore, Karnataka. A motivation for this study was the umerous reports about these polluted lakes and the occurrence of pathogenic icroorganisms in drinking water and the associated diseases. Water samples were collected from both the lakes in July 2017. PCR assay of 16S rRNA genes showed that the bacterial compositions of both lakes are largely similar. The present study identified 36 bacterial strains which include highly pathogenic bacterial species like Klebsiellapneumoniae, Escherichia coli, Staphylococcus aureus, Citrobacterfreundii, Enterobacteraerogenes, industrially important bacterial species such as Pseudomonas aeruginosa, Micrococcus luteus, Bacillus amyloliquifaciensand Bacillus subtilis This is the first report of molecular study ofbacteria from these lakes This study has clearly revealed that currently, the microbiological quality of Varthur and Bellandur water makes them unfit for drinking and recreational activities due to contamination by bacteria. The water analyzed in this study has clearly shown that they are loaded with the contaminants indicator organisms which are the indication of feacal pollution. This study helps to assess its usefulness as portable/ recreational water and to recommend control measures where necessary.
Authors and Affiliations
Smitha Murthy, Venkata Narasimha Kadali and Sheji Chandran N
Keywords
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