Comparison of phenotypic methods for the detection of extended spectrum beta lactamase production among enterobacteriaceae
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2016, Vol 21, Issue 1
Abstract
Introduction: Extended-spectrum β-lactamases (ESBLs) are enzymes which hydrolyse extended-spectrum Cephalosporins and are more common in recent times. Aim: We aimed to compare Chrome agar, double disk synergy test (DDST) and potentiated combined disk diffusion test (PCDDT) with ESBL E test for their sensitivity in ESBL detection. Materials and Methods: The study was a cross-sectional prospective analytical study involving 100 isolates of Enterobacteriaceae family between May 2014 and December 2014. All the isolates were subjected to ESBL detection by Chrome agar, double disk synergy test (DDST), Phenotypic confirmatory disk diffusion test (PCDDT) and ESBL Estrip method. Results: Among the 100 isolates tested, 61 isolates were resistant to one of the third generation Cephalosporins. Among them potentiated disk diffusion test (PCDDT) detected 30/61 isolates (49.18%), DDST detected26 (42.62%) and E strip detected detected35 (57.37%) isolates. Chrome agar showed growth on 42 samples. Conclusions: In resource poor settings, Chrome agar along with Phenotypic confirmatory disk diffusion test (PCDDT) can be taken as an effective screening method for ESBL detection.
Authors and Affiliations
M Anitha Raj, V Abarna, Arthi E
Keywords
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