A NEW RP-HPLC METHOD FOR THE SIMULATANEOUS ESTIMATION OF NEBIVOLOL AND VALSARTAN IN TABLETS
Journal Title: Indo American Journal of Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
A simple RP-HPLC method was developed and validated for the simultaneous estimation of Valsartan and Nebivolol in tablets. The Valsartan and Nebivolol were analyzed by RP-HPLC using Hypersil BDS column (150 cm X 4.6 mm, 5 μm) with isocratic mobile phase consists of ammonium acetate buffer to pH 5.20 and acetonitrile (55:45, v/v), at a flow rate of 1 ml/min in the detection wavelength of 282 nm. The linearity ranges of Valsartan are 200-1200 µg/ml and 12.5-75 µg/ml for Nebivolol by RP-HPLC. The limit of detection and quantification for Valsartan and Nebivolol were found to be 0.25 µg/ml and 1.4 µg/ml, 0.77 µg/ml and 4.5 µg/ml respectively. The accuracy of this method evaluated by recovery measurements and good recovery results obtained from 99.61% to 101.65% for all parameters and the relative standard deviation is below 2 % were achieved. Key words: Valsartan, Nebivolol, Estimation, Accuracy, Tablets.
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