A Non-Invasive Glucose Estimation in Saliva Samples by using Pre Column Derivatised RP-HPLC
Journal Title: International Journal of Diabetology & Vascular Disease Research (IJDVR) - Year 2016, Vol 4, Issue 1
Abstract
Diagnostic devices available in the market to measure blood glucose level which requires blood as diagnostic body fluid. Hence, necessity arises to find some non-invasive diagnostic mean to measure body glucose level frequently without any discomfort to the patient. The present study was aimed at estimation of blood and salivary glucose level in diabetic and non diabetic subjects. Saliva is one of the most abundant secretions in the human body and its collection is easy and noninvasive. The aim of this study was to find a medium that can be used to diagnose and monitor diabetes. In this, saliva could play a major role. To explain the role of saliva as a diagnostic tool, saliva samples were compared with blood glucose in healthy and diabetic subjects. 50 subjects were included in this study. The subjects were asked to come to the clinic in the early morning and after 2 hours fast. At that time, 5 mL of venous blood was collected in an ethylene diamine tetra acetic acid (EDTA)-containing blood collection tube for estimation. Unstimulated saliva was collected from subjects. The saliva and plasma from the blood samples were subjected to glucose estimation by pre-derivatised RP-HPLC method. A correlation was observed between FSG and FPG of diabetic as well as non diabetic subjects. The correlation coefficients for non-diabetic and diabetic subjects were 0.9967, proving the correlation between fasting saliva glucose and fasting blood glucose values statistically. Values observed regarding blood and saliva glucose level were found distinctly different between normal and diabetic subjects suggesting that monitoring of saliva glucose level can be used as an index of diabetes mellitus.
Authors and Affiliations
P. V Suresh
Keywords
Related Articles
- EP ID EP198841
- DOI 10.19070/2328-353X-1600031
- Views 119
- Downloads 0
Diabetes Mellitus And The Brain: Special Emphasis To Cognitive Function
Diabetes mellitus (DM) is a major public health problem. Cognitive deficits are common with DM which range from subclinical or subtle to severe deficits as dementia. Both hypoglycemia and hyperglycemia are causes of cogn...
Pharmacological Properties of Capparis spinosa Linn
Capparis spinosa Linn. (Capparaceae) was traditionally used for pharmacological purposes and has potential for use in modern cosmetics. This review aims to assess the current available knowledge of Capparis spinosa and i...
The Attitudes about Complex Therapy Scale (ACTS) in Type 2 Diabetes and Cardiovascular Disease: Development, Validity and Reliability
Background: Type 2 diabetes is associated with cardiovascular disease, and patients with both conditions are prescribed complex medication regimens. Aim: The aim was to develop a reliable and valid measure of attitudes a...
A Non-Invasive Glucose Estimation in Saliva Samples by using Pre Column Derivatised RP-HPLC
Diagnostic devices available in the market to measure blood glucose level which requires blood as diagnostic body fluid. Hence, necessity arises to find some non-invasive diagnostic mean to measure body glucose level fre...
Magnesium Deficiency Results in Oxidation and Fragmentation of DNA, Down Regulation of Telomerase Activity, and Ceramide Release in Cardiovascular Tissues and Cells: Potential Relationship to Atherogenesis, Cardiovascular Diseases and Aging
There is a growing awareness that dietary deficiency of magnesium is becoming a serious problem, particularly in the Western World. Disturbances in diet are known to promote lipid deposition in the arterial walls and acc...