A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics
Journal Title: Journal of Clinical and Medical Research - Year 2020, Vol 2, Issue 2
Abstract
Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.
Authors and Affiliations
Ingridi de Souza Sene1,2, Vladimir Costa2, Debora Cavalcante Braz2,3, Emanuel Airton de Oliveira Farias4, Gisele Elias Nunes5, Ivan H Bechtold5, Livio Cesar Cunha Nunes3, Carla Eiras6* and Carlos Henrique Nery Costa1,2
Keywords
Related Articles
- EP ID EP708386
- DOI https://doi.org/10.37191/Mapsci-2582-4333-2(2)-032
- Views 67
- Downloads 0
PsychoSocial Impact of Covid-19 on Saudi Children
Aim: With over 60 million recorded cases, and the death of 2 million the world is indeed struggling with a serious public health threat, coronavirus. Lack of health awareness and directive particularly for children. Heal...
Characterization of Patients in Fertile Age with Vaginal Flow Syndrome:Consultation No. 10. Plinlinico Pàrraga
Introduction: Vaginal discharge syndrome is an infectious process of the vagina with a florid symptomatology and psychosocial impact on the female population. Objective: To characterize the patients of childbearing ag...
International Reactions to COVID-19
The recent outbreak of the 2019 novel coronavirus in China, which was tentatively named 2019-nCoV, and now officially declared as COVID-19 by the World Health Organization (WHO), [1] has caused a lot of panic around the...
Anaesthesia Concerns in Apert Syndrome - A Case Report
Apert syndrome (AS) a type of acrocephalosyndactyly is a rare congenital disorder with the autosomal dominant mode of transmission that consists of craniofacial synostosis, midfacial hypoplasia, and bilateral limb syndac...
Development of Animal Models, Presenting Appropriate Analogues to Respective Human Systems
The main idea of the current study was directed to developed appropriate experimental animal models, imitating respective systems with the human origin, and giving a possibility when the last is not available, experiment...