Development of Animal Models, Presenting Appropriate Analogues to Respective Human Systems
Journal Title: Journal of Clinical and Medical Research - Year 2021, Vol 3, Issue 1
Abstract
The main idea of the current study was directed to developed appropriate experimental animal models, imitating respective systems with the human origin, and giving a possibility when the last is not available, experiments about necessary applications to humans to be performed. So, an additional copy of oncogene Dcn1 in normal mouse embryonic stem cells (mESCs), was inserted, by appropriate recombinant DNA-constructs, based on the AAV DNA-genome. All derived genetically-manipulated cellular types were co-cultivated with early myeloid and lymphoid progenitors, derived from non-transfected mESCs in the presence of GM-CSF (for induction of initial stages of both myeloid and lymphoid differentiation), and subsequently, malignant antigens were added (about further phagocyte and plasmatic cell differentiation, respectively). The derived and selected mESCs, containing an additionally-inserted copy of oncogene Dcn1, which indicated preserved normal/non-malignant characteristics both in vitro and in vivo, presented appropriate experimental normal mouse cellular analog of the cited in the scientific literature human embryonic trophoblasts, immortalized by infection with virus SV40. Additionally, the results obtained showed a possibility about the expression of membrane receptor glycoproteins by non-lymphoid and non-myeloid cellular types inappropriate conditions. Also, the presented study demonstrated the importance of the blood-testes barrier (BTB) for the prevention of malignancy development in the experimental hamster Graffi tumor model. The role of bio-molecules, as well as of intra- and extra-cellular inter-molecular interactions in cascade regulatory mechanisms, inactivation of the differentiation of embryonic and adult stem/progenitor cells in normal types, as well as for suppression of malignant transformation, was suggested. The established analogy of the developed and investigated in the current study experimental animal models gives a possibility for their application about performing of specific experiments when the respective systems with human origin are not available.
Authors and Affiliations
Iskra V Sainova*, Iliana N Ilieva*, RenetaToshkova, Ani Georgieva and Liliya Georgieva*
Keywords
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