A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2013, Vol 7, Issue 2
Abstract
The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 µm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 0C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision.
Authors and Affiliations
Violeta Giugiu
Keywords
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