Advanced glycation end products in the plasma and renal tissues in experimental model of unilateral renal artery stenosis
Journal Title: Progress in Health Sciences - Year 2012, Vol 2, Issue 2
Abstract
Purpose: To evaluate whether advanced glycation end products (AGE) levels are increased in the plasma and renal tissues of rats after unilateral renal artery stenosis (RAS). Materials and methods: AGE levels were measured using commercially available ELISA kit in plasma and renal tissue samples obtained from 16 rats with experimental induced RAS for 3 and 28 days and from 6 respective sham-operated control rats. We also analyzed by HPLC the concentration of 4-hydroxynonenal (4-HNE), a known inducer of AGE formation and accumulation. Results: Plasma concentrations of 4-HNE and AGE were significantly increased (p<0.05) after 28 days of RAS. At this time point, the concentration of AGE was markedly increased in the clipped atrophic kidney (by about 10-fold; p<0.05), but it was unchanged in the contralateral kidney of the same rats. No differences in plasma and renal AGE levels were detected at day 3 of RAS. Sham operation did not affect the levels of AGE and 4-HNE at each time point. Conclusions: Increased accumulation of AGE both in the plasma and in the ischemic atrophic kidney suggest that AGE levels can be used as a reliable biomarker for monitoring the development of ischemic nephropathy caused by renal artery stenosis.
Authors and Affiliations
J Brańska, K Kruszewski, I Jarocka, A Tarasik, H Ostrowska
Keywords
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