AN OVERVIEW ON HIGH PERFORMANCE LIQUIDCHROMATOGRAPHY- MASS SPECTROSCOPY
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 5
Abstract
Ultra high performance liquid chromatography\ms is an extremely versatile instrumental technique. As the name suggested the instrumentation comprises a high performance liquid chromatography attached, via suitable interface, to a mass spectrometer. The primary advantage UHPLC/MS has over GC/MS is that it is capable of analyzing a much wider range of components. Compounds that are thermally liable, exhibit high polarity or have a high molecular mass may all be analyzed using UHPLC/MS even proteins may be routinely analyzed. Ultra high performance liquid chromatography (UHPLC) performs separations 5 to 10 times faster than conventional HPLC by employing sub-2 μm diameter particles. The 1-2 second peak widths and relatively high separation efficiency of UHPLC are more competitive with capillary GC, making UHPLC-MS an attractive alternative method for illicit drug analysis. The columns packed with porous sub-2μm particles and the extension of the upper pressure limit of HPLC instrumentation to 1300bar (ultra-high pressure liquid chromatography, UHPLC) has opened new frontiers in resolution and speed of analysis. However, certain constraints appear when coupling UHPLC technology with mass spectrometry (MS). Firs Chromatography (HPLC) directly coupled to mass spectrometry (MS) was in routine use in drug metabolism laboratories. It can give Enhanced selectivity and sensitivity, and rapid, generic analysis
Authors and Affiliations
Navya Sri D* , Ramamohan Reddy T1 , Ajitha A1 , Uma Maheshwara Rao. V1
Keywords
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