AN OVERVIEW ON HPLC, UPLC AND ITS TROUBLESHOOTING
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4
Abstract
HPLC and UPLC Systems take advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. When taken together, these achievements have created a stepfunction improvement in chromatographic performance.Although HPLC method development has been improved by advances in column technology and instrumentation, problems still arise. This helps in systematic means of isolating, identifying, and correcting many typical problems. The important segments of an HPLC and UPLC system are the same, whether you use a modular system or a more sophisticated unit except the column characteristics. Problems affecting overall system performance can arise in each component. Solutions to these problems are presented in easy-to-use tables.
Authors and Affiliations
Nidhi Nk* , Neelam I , AjithaA , Uma Maheswar Rao V
Keywords
Related Articles
- EP ID EP90307
- DOI -
- Views 174
- Downloads 0
SPECTROPHOTOMETRIC ESTIMATION OF CEFPROZIL IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS
Two simple, accurate, rapid and sensitive spectrophotometric methods (A and B) have been developed for estimation of cefprozil in bulk drug and pharmaceutical formulations. The methods A and B were based on oxidati...
ANALYTICAL TECHNIQUES FOR THE ESTIMATION OFAGOMELATINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS: A REVIEW
Agomelatine (N-[2-(7-methoxynaphthalen-1-yl) ethyl] acetamide), its antidepressant efficacy has been verified in the treatment of major depressive disorder (MDD).Agomelatine showed significant benefits over paroxetine du...
BINARY RESPONSES OF RETINOPATHY IN T2DM USING GEE MODELING AND GOODNESS OF FIT TESTS
ABSTRACT Retinopathy is a major cause of blindness in DM worldwide. We aimed to study the prevalence of retinopathy in DM by screening and its complications. The risk factors are uncontrolled DM prolonged durati...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION AND STABILITY STUDIES OF THE ESTIMATION OF AGOMELATINE IN TABLET DOSAGE FORM BY RP-HPLC
Agomelatine is a new melatonergic antidepressant with a unique pharmacological action. A stability indicating RP-HPLC method was developed and validated for the determination of agomelatine in active pharmaceutical ingre...
A REVIEW ON AUGER ELECTRON SPECTROSCOPY
The Auger electron energies can be defined as the characteristics of the element from which the electron come. Auger electron Spectroscopy (AES) measures the energies of Auger electrons by identifying surface eleme...